Product nameAnti-YY1 antibody
See all YY1 primary antibodies
DescriptionRabbit polyclonal to YY1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human YY1 aa 1-50. The exact sequence is proprietary. NP_003394.1
Database link: P25490
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245364 was affinity purified using an epitope specific to YY1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245364 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 45 kDa.|
FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Sequence similaritiesBelongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.
Cellular localizationNucleus matrix. Associated with the nuclear matrix.
- Information by UniProt
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
All lanes : Anti-YY1 antibody (ab245364) at 0.04 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30 seconds
YY1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245364 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245364 at 1 µg/ml.
Lane 1: ab245364 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
ab245364 has not yet been referenced specifically in any publications.