Overview

  • Product name

  • Description

    Rabbit polyclonal to YY1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human YY1 aa 1-50. The exact sequence is proprietary. NP_003394.1
    Database link: P25490

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.

Target

  • Function

    Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • Sequence similarities

    Belongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Images

  • All lanes : Anti-YY1 antibody (ab245364) at 0.04 µg

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa


    Exposure time: 30 seconds
  • YY1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245364 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245364 at 1 µg/ml.

    Lane 1: ab245364 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

References

ab245364 has not yet been referenced specifically in any publications.

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