Product nameAnti-YY1 antibody [EPR4651]
See all YY1 primary antibodies
DescriptionRabbit monoclonal [EPR4651] to YY1
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Unsuitable for: ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human YY1 aa 50-150. The exact sequence is proprietary.
- Purchase matching WB positive control:Recombinant Human YY1 protein
- Human colon tissue; HeLa, Y79 and HuT-78 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 45 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Sequence similaritiesBelongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.
Cellular localizationNucleus matrix. Associated with the nuclear matrix.
- Information by UniProt
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
All lanes : Anti-YY1 antibody [EPR4651] (ab109228) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Y79 cell lysates
Lane 3 : HuT-78 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Yang T et al. YY1: A novel therapeutic target for diabetic nephropathy orchestrated renal fibrosis. Metabolism 96:33-45 (2019). Read more (PubMed: 31028762) »
- Liu X et al. Metastatic breast cancer cells overexpress and secrete miR-218 to regulate type I collagen deposition by osteoblasts. Breast Cancer Res 20:127 (2018). Read more (PubMed: 30348200) »