Our Abpromise guarantee covers the use of ab109228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
    • Sequence similarities

      Belongs to the YY transcription factor family.
      Contains 4 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus matrix. Associated with the nuclear matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • CF1 antibody
      • Delta antibody
      • Delta transcription factor antibody
      • INO80 complex subunit S antibody
      • INO80S antibody
      • NF E1 antibody
      • NF-E1 antibody
      • NFE1 antibody
      • OTTHUMP00000197459 antibody
      • Transcriptional repressor protein YY1 antibody
      • TYY1_HUMAN antibody
      • UCR motif DNA binding protein antibody
      • UCRBP antibody
      • Yin and yang 1 antibody
      • Yin and Yang 1 protein antibody
      • Yin Yang 1 antibody
      • Ying Yang 1 antibody
      • YY 1 antibody
      • YY 1 transcription factor antibody
      • YY-1 antibody
      • YY1 antibody
      • YY1 transcription factor antibody
      see all


    • All lanes : Anti-YY1 antibody [EPR4651] (ab109228) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Y79 cell lysates
      Lane 3 : HuT-78 cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


    This product has been referenced in:

    • Yang T  et al. YY1: A novel therapeutic target for diabetic nephropathy orchestrated renal fibrosis. Metabolism 96:33-45 (2019). Read more (PubMed: 31028762) »
    • Liu X  et al. Metastatic breast cancer cells overexpress and secrete miR-218 to regulate type I collagen deposition by osteoblasts. Breast Cancer Res 20:127 (2018). Read more (PubMed: 30348200) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109228.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up