Overview

  • Product name

    Anti-YY1 antibody [EPR4651] - BSA and Azide free
    See all YY1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4651] to YY1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IP, WBmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human YY1 aa 50-150. The exact sequence is proprietary.

  • General notes

    Ab239956 is the carrier-free version of ab109228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
    • Sequence similarities

      Belongs to the YY transcription factor family.
      Contains 4 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus matrix. Associated with the nuclear matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • CF1 antibody
      • Delta antibody
      • Delta transcription factor antibody
      • INO80 complex subunit S antibody
      • INO80S antibody
      • NF E1 antibody
      • NF-E1 antibody
      • NFE1 antibody
      • OTTHUMP00000197459 antibody
      • Transcriptional repressor protein YY1 antibody
      • TYY1_HUMAN antibody
      • UCR motif DNA binding protein antibody
      • UCRBP antibody
      • Yin and yang 1 antibody
      • Yin and Yang 1 protein antibody
      • Yin Yang 1 antibody
      • Ying Yang 1 antibody
      • YY 1 antibody
      • YY 1 transcription factor antibody
      • YY-1 antibody
      • YY1 antibody
      • YY1 transcription factor antibody
      see all

    Images

    • Immunohistochemical analysis of YY1 in paraffin embedded Human colon tissue, using a 1/250 dilution of ab109228.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109228).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab239956 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239956.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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