RecombinantRabMAb

Recombinant Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

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Flow Cytometry - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
  • Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
  • Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
  • Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4652] to YY1 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-YY1 antibody [EPR4652] - BSA and Azide free
    See all YY1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4652] to YY1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: ChIP or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human YY1 aa 250-350 (internal sequence). The exact sequence is proprietary.
    Database link: P25490

  • Positive control

    • IHC-P: Human cervix carcinoma tissue.

  • General notes

    Ab232573 is the carrier-free version of ab109237. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232573 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab232573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ChIP or IP.

    • Target

    • Function

      Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.

    • Sequence similarities

      Belongs to the YY transcription factor family.
      Contains 4 C2H2-type zinc fingers.

    • Cellular localization

      Nucleus matrix. Associated with the nuclear matrix.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CF1 antibody
      • Delta antibody
      • Delta transcription factor antibody
      • INO80 complex subunit S antibody
      • INO80S antibody
      • NF E1 antibody
      • NF-E1 antibody
      • NFE1 antibody
      • OTTHUMP00000197459 antibody
      • Transcriptional repressor protein YY1 antibody
      • TYY1_HUMAN antibody
      • UCR motif DNA binding protein antibody
      • UCRBP antibody
      • Yin and yang 1 antibody
      • Yin and Yang 1 protein antibody
      • Yin Yang 1 antibody
      • Ying Yang 1 antibody
      • YY 1 antibody
      • YY 1 transcription factor antibody
      • YY-1 antibody
      • YY1 antibody
      • YY1 transcription factor antibody
      see all

    Images

    • Flow Cytometry - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Flow Cytometry - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

      ab109237 staining YY1 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).

    • Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

      Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Immunocytochemistry/ Immunofluorescence - Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109237).

    • Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)
      Anti-YY1 antibody [EPR4652] - BSA and Azide free (ab232573)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab232573? Please let us know so that we can cite the reference in this datasheet.

    ab232573 has not yet been referenced specifically in any publications.

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