Recombinant Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seq
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-YY1 antibody [EPR4652] - Nuclear Loading Control
See all YY1 primary antibodies -
Description
Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra), ChIC/CUT&RUN-seqmore details
Unsuitable for: ChIP or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Daudi, Y79, and HuT-78 cell lysates, mouse and rat heart tissue. IHC-P: Human kidney, tonsil and cervix carcinoma tissues. ICC/IF: HeLa and HUT-78 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4652 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (7) |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
ICC/IF |
1/50.
For unpurified use at 1/100 - 1/250. |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. |
Target
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Function
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. -
Sequence similarities
Belongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers. -
Cellular localization
Nucleus matrix. Associated with the nuclear matrix. - Information by UniProt
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Database links
- Entrez Gene: 7528 Human
- Entrez Gene: 22632 Mouse
- Entrez Gene: 24919 Rat
- Omim: 600013 Human
- SwissProt: P25490 Human
- SwissProt: Q00899 Mouse
- Unigene: 388927 Human
- Unigene: 3868 Mouse
see all -
Alternative names
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
see all
Images
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ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/µL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of ab109237 [EPR4652]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/10000 dilution (purified)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling YY1 with purified ab109237 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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ab109237 staining YY1 in the human cell line HeLa (Human epithelial cell line from cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/30. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black).
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry/Immunofluorescence analysis of HUT-78 cells labelling YY1 with purified ab109237 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
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All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/50000 dilution (purified)
Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 2 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/2000 dilution (purified)
Lane 1 : Mouse heart
Lane 2 : Rat heart
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (ab109237) at 1/1000 dilution (unpurified)
Lane 1 : Daudi (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 3 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling YY1 with unpurified ab109237 at 1/100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human tonsil tissue labelling YY1 with unpurified ab109237 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labelling YY1 with unpurified ab109237 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab109237 has been referenced in 44 publications.
- Zheng Y et al. LncNAP1L6 activates MMP pathway by stabilizing the m6A-modified NAP1L2 to promote malignant progression in prostate cancer. Cancer Gene Ther 30:209-218 (2023). PubMed: 36195720
- Xu J et al. miR-301a Deficiency Attenuates the Macrophage Migration and Phagocytosis through YY1/CXCR4 Pathway. Cells 11:N/A (2022). PubMed: 36552718
- Ramirez RN et al. FoxP3 associates with enhancer-promoter loops to regulate Treg-specific gene expression. Sci Immunol 7:eabj9836 (2022). PubMed: 35030035
- Dong X et al. YY1 safeguard multidimensional epigenetic landscape associated with extended pluripotency. Nucleic Acids Res 50:12019-12038 (2022). PubMed: 35425987
- Liang S et al. Inhibition of NLRC5 attenuates the malignant growth and enhances the sensitivity of gastric cancer cells to 5-FU chemotherapy by blocking the carcinogenic effect of YY1. Exp Ther Med 24:601 (2022). PubMed: 35949331