Product nameAnti-YY1 antibody [EPR4652] - Nuclear Loading Control (HRP)
See all YY1 primary antibodies
DescriptionRabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human YY1 aa 250-350 (internal sequence). The exact sequence is proprietary.
- WB: HeLa and Daudi whole cell lysates. IHC-P: FFPE Human Colon Normal
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab199998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMultifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Sequence similaritiesBelongs to the YY transcription factor family.
Contains 4 C2H2-type zinc fingers.
Cellular localizationNucleus matrix. Associated with the nuclear matrix.
- Information by UniProt
- CF1 antibody
- Delta antibody
- Delta transcription factor antibody
IHC image of YY1 staining in a section of formalin-fixed paraffin-embedded nomal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab199998 at 1/1000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (HRP) (ab199998) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lys
Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199998 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
This product has been referenced in:
- Popp I et al. Fanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missense mutation in XPF. BMC Med Genet 19:7 (2018). Read more (PubMed: 29325523) »
- Weintraub AS et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell 171:1573-1588.e28 (2017). Read more (PubMed: 29224777) »