Recombinant
RabMAb

Recombinant Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (HRP) (ab199998)

Overview

  • Product name

    Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (HRP)
    See all YY1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4652] to YY1 - Nuclear Loading Control (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human YY1 aa 250-350 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and Daudi whole cell lysates. IHC-P: FFPE Human Colon Normal
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • Sequence similarities

    Belongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • CF1 antibody
    • Delta antibody
    • Delta transcription factor antibody
    • INO80 complex subunit S antibody
    • INO80S antibody
    • NF E1 antibody
    • NF-E1 antibody
    • NFE1 antibody
    • OTTHUMP00000197459 antibody
    • Transcriptional repressor protein YY1 antibody
    • TYY1_HUMAN antibody
    • UCR motif DNA binding protein antibody
    • UCRBP antibody
    • Yin and yang 1 antibody
    • Yin and Yang 1 protein antibody
    • Yin Yang 1 antibody
    • Ying Yang 1 antibody
    • YY 1 antibody
    • YY 1 transcription factor antibody
    • YY-1 antibody
    • YY1 antibody
    • YY1 transcription factor antibody
    see all

Images

  • IHC image of YY1 staining in a section of formalin-fixed paraffin-embedded nomal human colon*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab199998 at 1/1000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. 

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-YY1 antibody [EPR4652] - Nuclear Loading Control (HRP) (ab199998) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lys
    Lane 2 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199998 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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