Anti-z DNA antibody (ab2079)

Having checked with the lab, we can confirm that a preparation of Z-DNA (i.e. left-handed DNA) of sufficient purity has been injected.
I am sorry that we are unable to provide more details, but I hope this information is nevertheless helpful to you. We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

Thank you for contacting us and your patience.
Unfortunately, the antigen used to produce this product was made a few years ago in a laboratory at a private university. Most information regarding this antibody is propriety, however, I can tell you the following: Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen.
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Thank you for your inquiry. The lab where this antibody is produced informed me that, unfortunately, at this time they do not have a specific DNA sequence that could be used as a positive control for immunoprecipitation. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

Thank you for contacting us. Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen. I asked the lab about a recommended positive control. I will contact you again as soon as I receive this information.

Thank you for your enquiry. Your question is indeed an interesting one. The two most commonly used techniques for the preparation of chromatin are sonication and a mild micrococcal nuclease digestion. They can both be employed for use in cross linked chromatin. However, the nuclease digestion of chromatin relies on the fact that the digestion of DNA is obscured by the association of the DNA with the nucleosome template and therefore the chromatin is preferentially digested into mono, di- and tri-nucleosomes etc. However, given that you are digesting free Z-DNA this is not applicable as either MNase (or DNAse I or II) will fully digest the DNA to completion. The only thing that will provide you with suitable resolution is a restriction enzyme. This has been adopted in the context of chromatin in the following publication using a mild PstI digestion; Kang SH, Vieira K, Bungert J. 2002 Combining chromatin immunoprecipitation and DNA footprinting: a novel method to analyze protein-DNA interactions in vivo. Nucleic Acids Res. May 15;30(10) I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. There is a reference on the datasheet for this antibody(ab2079) which as far as I can tell recommends a dilution of 1:10 for ISH. European Journal of Histochemistry. 2004 48 (1) 49-56. Z-DNA a new in situ marker for transcription. I hope this reference will be helpful. If you are using the antibody for IP, then I would recommend using 10-500 µg cell lysate plus 0.5-5 µg of antibody. The precise amount will need to be optimised for your particular application. I hope this is helpful. Please do not hesitate to contact us again if you require more assistance.