Overview

  • Product name
    Anti-z DNA antibody
  • Description
    Sheep polyclonal to z DNA
  • Host species
    Sheep
  • Tested applications
    Suitable for: IP, In situ hybridizationmore details
  • Immunogen

    Z-DNA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.5
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
In situ hybridization Use at an assay dependent dilution. See below; Cerna, A. et al, European journal of Histochemistry.

Target

  • Relevance
    Z-DNA is one of the many possible double helical structures of DNA. It is a left-handed double helical structure in which the double helix winds to the left in a zig-zag pattern (instead of to the right, like the more common B-DNA form). Z-DNA is thought to be one of three biologically active double helical structures along with A- and B-DNA.

References

This product has been referenced in:
  • Leonova K  et al. TRAIN (Transcription of Repeats Activates INterferon) in response to chromatin destabilization induced by small molecules in mammalian cells. Elife 7:N/A (2018). Read more (PubMed: 29400649) »
  • Safina A  et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res 45:1925-1945 (2017). Read more (PubMed: 28082391) »

See all 4 Publications for this product

Publishing research using ab2079? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

6 reviews or Q&A

10
Votes: Highest First

Thanks for your reply.
I appreciate that information regarding the antibody is proprietary, I am not seeking disclosure of their methods. However, I still need to know if they carried out any testing on the antigen to establish its purity. In othe...

Read More

Having checked with the lab, we can confirm that a preparation of Z-DNA (i.e. left-handed DNA) of sufficient purity has been injected.
I am sorry that we are unable to provide more details, but I hope this information is nevertheless helpful to yo...

Read More

Please provide details on the method and the quality control used in the synthesis of the brominated DNA used to raise this antibody. Is the starting material removed from the bromination product prior to further use? Are DNA breakdown products removed...

Read More

Thank you for contacting us and your patience.
Unfortunately, the antigen used to produce this product was made a few years ago in a laboratory at a private university. Most information regarding this antibody is propriety, however, I can tell you...

Read More

I have purchased Z-DNA antibody(ab2079) and have two questions: - What is the immunogen for this antibody - Are you aware of a DNA sequence that could be used as a positive control for immuprecipitation Many thanks in advance

Thank you for your inquiry. The lab where this antibody is produced informed me that, unfortunately, at this time they do not have a specific DNA sequence that could be used as a positive control for immunoprecipitation. We will updat...

Read More

I have purchased Z-DNA antibody(ab2079) and have two questions: - What is the immunogen for this antibody - Are you aware of a DNA sequence that could be used as a positive control for immuprecipitation Many thanks in advance

Thank you for contacting us. Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen. I asked the lab about a recommended positive control. I will contact you...

Read More

I have purchased Z-DNA antibody(ab2079), and then crosslinked it to Z-DNA using formaldehyde. Would you give me some suggestuions about how to shear the cross-linked DNA to 500-1000 base pairs ? By the way, the sonication treatment is not allowed in th...

Read More

Thank you for your enquiry. Your question is indeed an interesting one. The two most commonly used techniques for the preparation of chromatin are sonication and a mild micrococcal nuclease digestion. They can both be employed for use in cross link...

Read More

I have ordered the Z-DNA antibody(ab2079),but I don't know the effective final concentration.Do you know the binding plateau of the antibody? Thanks.

Thank you for your enquiry. There is a reference on the datasheet for this antibody(ab2079) which as far as I can tell recommends a dilution of 1:10 for ISH. European Journal of Histochemistry. 2004 48 (1) 49-56. Z-DNA a new in situ marker for tran...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up