Anti-z DNA antibody (ab2079)

Reviews (1)Q&A (6)References (4)

Key features and details

  • Sheep polyclonal to z DNA
  • Suitable for: IP, In situ hybridization
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-z DNA antibody

  • Description

    Sheep polyclonal to z DNA

  • Host species

    Sheep

  • Tested applications

    Suitable for: IP, In situ hybridizationmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to z DNA conjugated to bovine serum albumin. Brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.50
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent dilution.
In situ hybridization
Use at an assay dependent dilution. See below; Cerna, A. et al, European journal of Histochemistry.
Notes
IP
Use at an assay dependent dilution.
In situ hybridization
Use at an assay dependent dilution. See below; Cerna, A. et al, European journal of Histochemistry.

Target

  • Relevance

    Z-DNA is one of the many possible double helical structures of DNA. It is a left-handed double helical structure in which the double helix winds to the left in a zig-zag pattern (instead of to the right, like the more common B-DNA form). Z-DNA is thought to be one of three biologically active double helical structures along with A- and B-DNA.

References (4)

Publishing research using ab2079? Please let us know so that we can cite the reference in this datasheet.

ab2079 has been referenced in 4 publications.

  • Leonova K  et al. TRAIN (Transcription of Repeats Activates INterferon) in response to chromatin destabilization induced by small molecules in mammalian cells. Elife 7:N/A (2018). PubMed: 29400649
  • Safina A  et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res 45:1925-1945 (2017). PubMed: 28082391
  • Lu P  et al. The Za domain of fish PKZ converts DNA hairpin with d(GC)n inserts to Z-conformation. Acta Biochim Biophys Sin (Shanghai) 45:1062-8 (2013). PubMed: 24113090
  • Cerná A  et al. Z-DNA, a new in situ marker for transcription. Eur J Histochem 48:49-56 (2004). PubMed: 15145775

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-z DNA antibody

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Testis)
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Dec 18 2020

Question

Thanks for your reply.
I appreciate that information regarding the antibody is proprietary, I am not seeking disclosure of their methods. However, I still need to know if they carried out any testing on the antigen to establish its purity. In other words, can the supplier state that only Z-DNA was injected?

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Answer

Having checked with the lab, we can confirm that a preparation of Z-DNA (i.e. left-handed DNA) of sufficient purity has been injected.
I am sorry that we are unable to provide more details, but I hope this information is nevertheless helpful to you. We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

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Question

Please provide details on the method and the quality control used in the synthesis of the brominated DNA used to raise this antibody. Is the starting material removed from the bromination product prior to further use? Are DNA breakdown products removed from the bromination products? Is the bromination done under controlled conditions? What temperature is the DNA bromination carried out under?

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Answer

Thank you for contacting us and your patience.
Unfortunately, the antigen used to produce this product was made a few years ago in a laboratory at a private university. Most information regarding this antibody is propriety, however, I can tell you the following: Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen.
I hope this information is still helpful for you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I have purchased Z-DNA antibody(ab2079) and have two questions: - What is the immunogen for this antibody - Are you aware of a DNA sequence that could be used as a positive control for immuprecipitation Many thanks in advance

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Answer

Thank you for your inquiry. The lab where this antibody is produced informed me that, unfortunately, at this time they do not have a specific DNA sequence that could be used as a positive control for immunoprecipitation. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

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Question

I have purchased Z-DNA antibody(ab2079) and have two questions: - What is the immunogen for this antibody - Are you aware of a DNA sequence that could be used as a positive control for immuprecipitation Many thanks in advance

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Answer

Thank you for contacting us. Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen. I asked the lab about a recommended positive control. I will contact you again as soon as I receive this information.

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Question

I have purchased Z-DNA antibody(ab2079), and then crosslinked it to Z-DNA using formaldehyde. Would you give me some suggestuions about how to shear the cross-linked DNA to 500-1000 base pairs ? By the way, the sonication treatment is not allowed in the current situation.

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Answer

Thank you for your enquiry. Your question is indeed an interesting one. The two most commonly used techniques for the preparation of chromatin are sonication and a mild micrococcal nuclease digestion. They can both be employed for use in cross linked chromatin. However, the nuclease digestion of chromatin relies on the fact that the digestion of DNA is obscured by the association of the DNA with the nucleosome template and therefore the chromatin is preferentially digested into mono, di- and tri-nucleosomes etc. However, given that you are digesting free Z-DNA this is not applicable as either MNase (or DNAse I or II) will fully digest the DNA to completion. The only thing that will provide you with suitable resolution is a restriction enzyme. This has been adopted in the context of chromatin in the following publication using a mild PstI digestion; Kang SH, Vieira K, Bungert J. 2002 Combining chromatin immunoprecipitation and DNA footprinting: a novel method to analyze protein-DNA interactions in vivo. Nucleic Acids Res. May 15;30(10) I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

I have ordered the Z-DNA antibody(ab2079),but I don't know the effective final concentration.Do you know the binding plateau of the antibody? Thanks.

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Answer

Thank you for your enquiry. There is a reference on the datasheet for this antibody(ab2079) which as far as I can tell recommends a dilution of 1:10 for ISH. European Journal of Histochemistry. 2004 48 (1) 49-56. Z-DNA a new in situ marker for transcription. I hope this reference will be helpful. If you are using the antibody for IP, then I would recommend using 10-500 µg cell lysate plus 0.5-5 µg of antibody. The precise amount will need to be optimised for your particular application. I hope this is helpful. Please do not hesitate to contact us again if you require more assistance.

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