Z-LEHD-FMK, caspase-9 inhibitor (ab142026)
Key features and details
- Irreversible caspase-9 inhibitor
- CAS Number: 210345-04-3
- Purity: > 98%
Supplied in DMSO (10 mM)
- Form / State: Liquid
- Source: Synthetic
Overview
-
Product name
Z-LEHD-FMK, caspase-9 inhibitor -
Description
Irreversible caspase-9 inhibitor -
Purity
> 98% -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 1074 Caspase-9 Inhibitor Z-LEHD-FMK. 1074-20C is the same size as the 20 µl size of ab142026.
-
CAS Number
210345-04-3 -
Chemical structure
Properties
-
Molecular weight
690.70 -
Molecular formula
C32H43FN6O10 -
Sequence
LEHD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Glu-2 = Glu(OMe); Asp-4 = Asp(OMe)) -
PubChem identifier
44135217 -
Storage instructions
Shipped at 4°C. Store at -20°C. Store under desiccating conditions. -
Solubility overview
Supplied in DMSO (10 mM)
-
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
See SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
CC(C)CC(C(=O)NC(CCC(=O)OC)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC2=CC=CC=C2 -
Source
Synthetic
-
Research areas
Images
-
2D chemical structure image of ab142026, Z-LEHD-FMK, caspase-9 inhibitor
-
Differentiated muscle cells were pre-incubated with 20 µM Z-LEHD-FMK (irreversible caspas-9 inhibitor) for 30 min before exposure to anoxic conditions. C) Light microscopy pictures demonstrates morphological changes during anoxia treatment. Arrows indicate rounded up, dead cells. D) Live/dead analysis demonstrate increased number of dead cells during 6 h anoxia, but this is unaffected by the irreversible caspase-9 inhibitor.
-
Functional assays: Caspase 9 Inhibitor Drug Detection Kit (ab102497)
Titration of the caspase 9 inhibitor Z-LEHD-FMK (ab142026) (duplicates; +/- SD).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab142026 has been referenced in 5 publications.
- Hou P et al. The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication. Autophagy 19:551-569 (2023). PubMed: 35670302
- Selkrig J et al. Spatiotemporal proteomics uncovers cathepsin-dependent macrophage cell death during Salmonella infection. Nat Microbiol 5:1119-1133 (2020). PubMed: 32514074
- Yang H et al. Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways. World J Gastrointest Oncol 11:377-392 (2019). PubMed: 31139308
- Rønning SB et al. Primary bovine skeletal muscle cells enters apoptosis rapidly via the intrinsic pathway when available oxygen is removed. PLoS One 12:e0182928 (2017). PubMed: 28792534
- Suzuki A et al. c-Jun N-terminal kinase-mediated Rubicon expression enhances hepatocyte lipoapoptosis and promotes hepatocyte ballooning. World J Gastroenterol 22:6509-19 (2016). PubMed: 27605885