Product nameZ-VDVAD-FMK, caspase-2 inhibitor
DescriptionIrreversible caspase-2 inhibitor
Biological descriptionIrreversible caspase-2 inhibitor. Inhibits apoptosis in multiple biological systems.
SequenceVDVAD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Asp-2 = Asp(OMe); Asp-5 = Asp(OMe))
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 20 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab142035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab13847 staining active caspase 3 in SKNSH cells treated with Z-VDVAD-FMK (ab142035), by ICC/IF. Decrease in active caspase 3 expression correlates with increased concentration of Z-VDVAD-FMK, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab142035 (Z-VDVAD-FMK) in DMSO. After this incupation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24 hours. The samples were then fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with
This product has been referenced in:
- Chauvier D et al. Upstream control of apoptosis by caspase-2 in serum-deprived primary neurons. Apoptosis 10:1243-59 (2005). Read more (PubMed: 16215683) »
- Robertson JD et al. Caspase-2 acts upstream of mitochondria to promote cytochrome c release during etoposide-induced apoptosis. J Biol Chem 277:29803-9 (2002). Read more (PubMed: 12065594) »
- Meguro T et al. Caspase inhibitors attenuate oxyhemoglobin-induced apoptosis in endothelial cells. Stroke 32:561-6 (2001). Read more (PubMed: 11157197) »