Z-VEID-FMK, caspase-6 inhibitor (ab142025)
Key features and details
- Potent, irreversible and cell-permeable caspase-6 inhibitor
- CAS Number:
- Purity: > 98%
- Supplied in DMSO (10 mM)
- Form / State: Liquid
- Source: Synthetic
Overview
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Product name
Z-VEID-FMK, caspase-6 inhibitor -
Description
Potent, irreversible and cell-permeable caspase-6 inhibitor -
Purity
> 98% -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 1011 Caspase-6 Inhibitor Z-VEID-FMK. 1011-20C is the same size as the 20 µl size of ab142025.
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Chemical structure
Properties
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Molecular weight
652.90 -
Molecular formula
C31H45FN4O10 -
Sequence
VEID (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Glu-2 = Glu(OMe); Asp-4 = Asp(OMe)) -
PubChem identifier
44135214 -
Storage instructions
Store at -20°C (desiccating conditions). -
Solubility overview
Supplied in DMSO (10 mM) -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CCC(C)C(C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CCC(=O)OC)NC(=O)C(C(C)C)NC(=O) OCC1=CC=CC=C1 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab142025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
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2D chemical structure image of ab142025, Z-VEID-FMK, caspase-6 inhibitor
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SHSY5Y cells were incubated at 37 °C for 1h with vehicle control (0 μM) and 1 μM of Z-VEID-FMK (ab142025). After this incubation 10µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-VEID-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab142025 has been referenced in 1 publication.
- Bakovic A et al. Inhibitors of Venezuelan Equine Encephalitis Virus Identified Based on Host Interaction Partners of Viral Non-Structural Protein 3. Viruses 13:N/A (2021). PubMed: 34452398