Product nameZ-VEID-FMK, caspase-6 inhibitor
DescriptionPotent, irreversible and cell-permeable caspase-6 inhibitor
Biological descriptionPotent, irreversible caspase-6 inhibitor (IC50 = 128.6 nM). Inhibits TNFα-induced apoptosis. Inhibits nuclear protein cleavage and fas-induced apoptosis in vitro. Cell-permeable.
SequenceVEID (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Glu-2 = Glu(OMe); Asp-4 = Asp(OMe))
Storage instructionsStore at -20°C (desiccating conditions).
Solubility overviewSupplied in DMSO (10 mM)
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab142025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
SHSY5Y cells were incubated at 37 °C for 1h with vehicle control (0 μM) and 1 μM of Z-VEID-FMK (ab142025). After this incubation 10µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-VEID-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Mintzer R et al. A whole cell assay to measure caspase-6 activity by detecting cleavage of lamin A/C. PLoS One 7:e30376 (2012). Read more (PubMed: 22253931) »
- Taimen P & Kallajoki M NuMA and nuclear lamins behave differently in Fas-mediated apoptosis. J Cell Sci 116:571-83 (2003). Read more (PubMed: 12508117) »
- Abdo M et al. Role of tumor necrosis factor-alpha and the modulating effect of the caspases in rat corpus luteum apoptosis. Biol Reprod 68:1241-8 (2003). Read more (PubMed: 12606464) »