Overview

  • Product name
    Z-YVAD-FMK, Irreversible caspase-1 inhibitor
  • Description
    Irreversible caspase-1 inhibitor. Cell-permeable.
  • Biological description
    Irreversible caspase-1 inhibitor. Anti-inflammatory agent. Active in vitro. Cell-permeable.
  • Purity
    > 98%
  • Chemical structure
    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab141388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • SHSY5Y cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-YVAD-FMK (ab141388). After this incubation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-YVAD-FMK concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development so

References

This product has been referenced in:
  • Matos TJ  et al. Release of IL-1beta via IL-1beta-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene. Mediators Inflamm 2005:131-8 (2005). Read more (PubMed: 16106098) »
  • Rozman-Pungercar J  et al. Inhibition of papain-like cysteine proteases and legumain by caspase-specific inhibitors: when reaction mechanism is more important than specificity. Cell Death Differ 10:881-8 (2003). Read more (PubMed: 12867995) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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