Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
- Datasheet
- References (4)
Overview
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Product name
Z-YVAD-FMK, Irreversible caspase-1 inhibitor -
Description
Irreversible caspase-1 inhibitor. Cell-permeable. -
Biological description
Irreversible caspase-1 inhibitor. Anti-inflammatory agent. Active in vitro. Cell-permeable. -
Purity
> 98% -
Chemical structure
Properties
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Molecular weight
630.70 -
Molecular formula
C31H39FN4O9 -
Sequence
YVAD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Asp-4 = Asp(OMe)) -
PubChem identifier
16760349 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 20 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)OCC2=CC=CC=C2 -
Source
Synthetic
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Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab141388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Other - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)Image from Migita K, et al. Plos One, 9(5), e96703. Fig 3a,; doi: 10.1371/journal.pone.0096703Neutrophils were stimulated with SAA (5 µg/ml) in the presence or absence of Z-YVAD-FMK for 8 h. After stimulation, supernatants were analyzed for IL-1 Β production using ELISA. Values represent the mean ± SD of two independent experiments. *p<0.001 compared to SAA-stimulated neutrophils. -
Functional Studies - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)SHSY5Y cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-YVAD-FMK (ab141388). After this incubation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-YVAD-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development so
References
This product has been referenced in:
- Matos TJ et al. Release of IL-1beta via IL-1beta-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene. Mediators Inflamm 2005:131-8 (2005). Read more (PubMed: 16106098) »
- Rozman-Pungercar J et al. Inhibition of papain-like cysteine proteases and legumain by caspase-specific inhibitors: when reaction mechanism is more important than specificity. Cell Death Differ 10:881-8 (2003). Read more (PubMed: 12867995) »
