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I am using this antibody in WB of transfected cells. My protocol:
Loaded 40ug protein
Block: 5% milk
1' ab - 1:500 overnight at 4oC
2' ab - working well with other primaries
I would like to try ab65249 as a replacement.
Asked on Jun 14 2012
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab65249.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Jun 14 2012