RecombinantRabMAb

Recombinant Anti-ZAN antibody [EPR11209] (ab170925)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11209] to ZAN
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ZAN antibody [EPR11209]
    See all ZAN primary antibodies

  • Description

    Rabbit monoclonal [EPR11209] to ZAN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide within Human ZAN aa 2050-2150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y493

  • Positive control

    • Mouse testis lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11209

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 306 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.

    • Tissue specificity

      In testis, primarily in haploid spermatids.

    • Sequence similarities

      Contains 1 EGF-like domain.
      Contains 3 MAM domains.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 5 VWFC domains.
      Contains 4 VWFD domains.

    • Domain

      The MAM domains probably mediate sperm adhesion to the zona pellucida.
      During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
      The VWFD domain 2 may mediate covalent oligomerization.

    • Cellular localization

      Cell membrane. Exclusively on the apical region of the sperm head.
    • Information by UniProt

    • Database links

      • Alternative names

        • OTTHUMP00000211358 antibody
        • OTTHUMP00000211359 antibody
        • OTTHUMP00000211360 antibody
        • OTTHUMP00000211361 antibody
        • OTTHUMP00000211362 antibody
        • OTTHUMP00000211363 antibody
        • ZAN antibody
        • ZAN_HUMAN antibody
        • Zonadhesin antibody
        see all

      Images

      • Western blot - Anti-ZAN antibody [EPR11209] (ab170925)
        Western blot - Anti-ZAN antibody [EPR11209] (ab170925)
        Anti-ZAN antibody [EPR11209] (ab170925) at 1/1000 dilution + Mouse testis lysate at 10 µg

        Secondary
        Goat anti-rabbit HRP conjugate at 1/2000 dilution

        Predicted band size: 306 kDa

      • Anti-ZAN antibody [EPR11209] (ab170925)
        Anti-ZAN antibody [EPR11209] (ab170925)

      References (0)

      Publishing research using ab170925? Please let us know so that we can cite the reference in this datasheet.

      ab170925 has not yet been referenced specifically in any publications.

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