Anti-ZAN antibody [EPR11209] (ab170925)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-ZAN antibody [EPR11209]
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DescriptionRabbit monoclonal [EPR11209] to ZAN
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Human
Does not react with: Rat -
Immunogen
Synthetic peptide within Human ZAN aa 2050-2150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y493 -
Positive control
- Mouse testis lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11209
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 306 kDa. |
Target
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FunctionBinds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.
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Tissue specificityIn testis, primarily in haploid spermatids.
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Sequence similaritiesContains 1 EGF-like domain.
Contains 3 MAM domains.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 5 VWFC domains.
Contains 4 VWFD domains. -
DomainThe MAM domains probably mediate sperm adhesion to the zona pellucida.
During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
The VWFD domain 2 may mediate covalent oligomerization. -
Cellular localizationCell membrane. Exclusively on the apical region of the sperm head.
- Information by UniProt
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Database links
- Entrez Gene: 7455 Human
- Entrez Gene: 22635 Mouse
- Omim: 602372 Human
- SwissProt: Q9Y493 Human
- SwissProt: O88799 Mouse
- Unigene: 307004 Human
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Alternative names
- OTTHUMP00000211358 antibody
- OTTHUMP00000211359 antibody
- OTTHUMP00000211360 antibody
see all
Images
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![Western blot - Anti-ZAN antibody [EPR11209] (ab170925)](https://www.abcam.com/ps/products/170/ab170925/Images/ab170925-171057-ab170925WB.jpg)
Western blot - Anti-ZAN antibody [EPR11209] (ab170925)Anti-ZAN antibody [EPR11209] (ab170925) at 1/1000 dilution + Mouse testis lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 306 kDa
Datasheets and documents
Protocols
References
ab170925 has not yet been referenced specifically in any publications.
