Recombinant
RabMAb

Recombinant Anti-ZAN antibody [EPR11209] (ab170925)

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Overview

  • Product name

    Anti-ZAN antibody [EPR11209]
    See all ZAN primary antibodies

  • Description

    Rabbit monoclonal [EPR11209] to ZAN

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide within Human ZAN aa 2050-2150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y493

  • Positive control

    • Mouse testis lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11209

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 306 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.

    • Tissue specificity

      In testis, primarily in haploid spermatids.

    • Sequence similarities

      Contains 1 EGF-like domain.
      Contains 3 MAM domains.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 5 VWFC domains.
      Contains 4 VWFD domains.

    • Domain

      The MAM domains probably mediate sperm adhesion to the zona pellucida.
      During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
      The VWFD domain 2 may mediate covalent oligomerization.

    • Cellular localization

      Cell membrane. Exclusively on the apical region of the sperm head.
    • Information by UniProt

    • Database links

      • Alternative names

        • OTTHUMP00000211358 antibody
        • OTTHUMP00000211359 antibody
        • OTTHUMP00000211360 antibody
        • OTTHUMP00000211361 antibody
        • OTTHUMP00000211362 antibody
        • OTTHUMP00000211363 antibody
        • ZAN antibody
        • ZAN_HUMAN antibody
        • Zonadhesin antibody
        see all

      Images

      • Western blot - Anti-ZAN antibody [EPR11209] (ab170925)
        Western blot - Anti-ZAN antibody [EPR11209] (ab170925)
        Anti-ZAN antibody [EPR11209] (ab170925) at 1/1000 dilution + Mouse testis lysate at 10 µg

        Secondary
        Goat anti-rabbit HRP conjugate at 1/2000 dilution

        Predicted band size: 306 kDa

      References

      ab170925 has not yet been referenced specifically in any publications.

      Publishing research using ab170925? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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