Key features and details
- Rabbit polyclonal to ZAP70 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZAP70 antibody - C-terminal
See all ZAP70 primary antibodies
DescriptionRabbit polyclonal to ZAP70 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ZAP70 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P43403
- WB: Jurkat cell lysate. IHC-P: Human tonsil tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab231452 in the following tested applications.
|WB||1/25. Predicted molecular weight: 69 kDa.
Incubate for 1 hour at room temperature.
Boil tissue section in citrate buffer, pH 6.0 for 10 minutes, followed by cooling at room temperature for 20 minutes.
Incubate with primary antibody for 30 minutes at room temperature.
FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificityExpressed in T- and natural killer cells.
Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
modificationsPhosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
- Information by UniProt
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 18.104.22.168 antibody
Anti-ZAP70 antibody - C-terminal (ab231452) at 1/25 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 69 kDa
Formalin-fixed, paraffin-embedded human tonsil tissue stained for ZAP70 using ab231452 at 1/100 dilution in immunohistochemical analysis.
ab231452 has not yet been referenced specifically in any publications.