Recombinant
Anti-ZAP70 antibody [E267] (ab32410)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-ZAP70 antibody [E267]
See all ZAP70 primary antibodies -
DescriptionRabbit monoclonal [E267] to ZAP70
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Host speciesRabbit
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Specificityab32410 recognises Zap-70, a Syk-family protein tyrosine kinase.
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Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
Unsuitable for: ICC or IHC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human ZAP70 aa 300-400 (N terminal). The exact sequence is proprietary.
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Positive control
- Jurkat cell lysate
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberE267
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/500. Predicted molecular weight: 70 kDa. | |
| IP | 1/30. |
Application notes
Is unsuitable for ICC or IHC.
Target
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FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
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Tissue specificityExpressed in T- and natural killer cells.
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Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains. -
DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
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Post-translational
modificationsPhosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. -
Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
- Information by UniProt
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Database links
- Entrez Gene: 7535 Human
- Entrez Gene: 22637 Mouse
- Entrez Gene: 301348 Rat
- Omim: 176947 Human
- SwissProt: P43403 Human
- SwissProt: P43404 Mouse
- Unigene: 234569 Human
- Unigene: 8038 Mouse
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Alternative names
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
Images
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![Western blot - Anti-ZAP70 antibody [E267] (ab32410)](https://www.abcam.com/ps/products/32/ab32410/Images/ab32410_1.JPG)
Western blot - Anti-ZAP70 antibody [E267] (ab32410)Anti-ZAP70 antibody [E267] (ab32410) at 1/500 dilution + Jurkat cell lysates
Predicted band size: 70 kDa
Observed band size: 70 kDa
Western blot analysis on Jurkat Cell lysate.
Datasheets and documents
References
This product has been referenced in:
- Liang W et al. Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation. Front Immunol 9:78 (2018). Read more (PubMed: 29434598) »
