RecombinantRabMAb

Recombinant Anti-ZAP70 antibody [E267] (ab32410)

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Western blot - Anti-ZAP70 antibody [E267] (ab32410)
  • Western blot - Anti-ZAP70 antibody [E267] (ab32410)
  • Anti-ZAP70 antibody [E267] (ab32410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E267] to ZAP70
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ZAP70 antibody [E267]
    See all ZAP70 primary antibodies

  • Description

    Rabbit monoclonal [E267] to ZAP70

  • Host species

    Rabbit

  • Specificity

    ab32410 recognises Zap-70, a Syk-family protein tyrosine kinase.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human ZAP70 aa 300-400 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500. Predicted molecular weight: 70 kDa.
Notes
WB
1/500. Predicted molecular weight: 70 kDa.
Application notes
Is unsuitable for ICC or IHC.

Target

  • Function

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

  • Tissue specificity

    Expressed in T- and natural killer cells.

  • Involvement in disease

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.

  • Domain

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

  • Cellular localization

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

  • Database links

    • Alternative names

      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC 2.7.10.2 antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain of T cell receptor associated protein kinase 70 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    Images

    • Western blot - Anti-ZAP70 antibody [E267] (ab32410)
      Western blot - Anti-ZAP70 antibody [E267] (ab32410)
      All lanes : Anti-ZAP70 antibody [E267] (ab32410) at 1/1000 dilution

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed)

      Predicted band size: 70 kDa



      Anti-GAPDH antibody, ab8245 (1/20000) was used as a primary antibody for the loading control and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed, ab216776 (1/10000) was used as a loading control secondary antibody.

      Lanes 1-2: Merged signal (red and green). Green – ab32410 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.
       ab32410 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

      The expression profile observed in Raji is consistent with the literature (PMID: 25275600).

      Negative control: Raji (PMID: 25275600)

    • Western blot - Anti-ZAP70 antibody [E267] (ab32410)
      Western blot - Anti-ZAP70 antibody [E267] (ab32410)
      Anti-ZAP70 antibody [E267] (ab32410) at 1/500 dilution + Jurkat cell lysates

      Predicted band size: 70 kDa
      Observed band size: 70 kDa



      Western blot analysis on Jurkat Cell lysate.
    • Anti-ZAP70 antibody [E267] (ab32410)
      Anti-ZAP70 antibody [E267] (ab32410)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab32410? Please let us know so that we can cite the reference in this datasheet.

    ab32410 has been referenced in 3 publications.

    • Han L  et al. IgD-Fc-Ig fusion protein, a new biological agent, inhibits T cell function in CIA rats by inhibiting IgD-IgDR-Lck-NF-?B signaling pathways. Acta Pharmacol Sin 41:800-812 (2020). PubMed: 31937932
    • Schleiss C  et al. BCR-associated factors driving chronic lymphocytic leukemia cells proliferation ex vivo. Sci Rep 9:701 (2019). PubMed: 30679590
    • Liang W  et al. Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation. Front Immunol 9:78 (2018). PubMed: 29434598

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