Recombinant
RabMAb

Recombinant Anti-ZAP70 antibody [E267] (ab32410)

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Overview

  • Product name

    Anti-ZAP70 antibody [E267]
    See all ZAP70 primary antibodies

  • Description

    Rabbit monoclonal [E267] to ZAP70

  • Host species

    Rabbit

  • Specificity

    ab32410 recognises Zap-70, a Syk-family protein tyrosine kinase.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ZAP70 aa 300-400 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 70 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for ICC or IHC.

    • Target

    • Function

      Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

    • Tissue specificity

      Expressed in T- and natural killer cells.

    • Involvement in disease

      Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.

    • Domain

      The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

    • Post-translational
      modifications

      Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

    • Cellular localization

      Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
    • Information by UniProt

    • Database links

      • Alternative names

        • 70 kDa zeta associated protein antibody
        • 70 kDa zeta-associated protein antibody
        • EC 2.7.10.2 antibody
        • FLJ17670 antibody
        • FLJ17679 antibody
        • Selective T cell defect antibody
        • SRK antibody
        • STD antibody
        • Syk related tyrosine kinase antibody
        • Syk-related tyrosine kinase antibody
        • Truncated ZAP kinase antibody
        • Tyrosine protein kinase ZAP70 antibody
        • Tyrosine-protein kinase ZAP-70 antibody
        • TZK antibody
        • ZAP 70 antibody
        • ZAP70 antibody
        • ZAP70_HUMAN antibody
        • Zeta chain associated protein kinase 70kD antibody
        • Zeta chain associated protein kinase 70kDa antibody
        • Zeta chain associated protein kinase 70kDa isoform 1 antibody
        • Zeta chain associated protein kinase 70kDa isoform 2 antibody
        • Zeta chain of T cell receptor associated protein kinase 70 antibody
        • Zeta chain TCR associated protein kinase 70kD antibody
        • Zeta chain TCR associated protein kinase 70kDa antibody
        see all

      Images

      • Western blot - Anti-ZAP70 antibody [E267] (ab32410)
        Western blot - Anti-ZAP70 antibody [E267] (ab32410)
        Anti-ZAP70 antibody [E267] (ab32410) at 1/500 dilution + Jurkat cell lysates

        Predicted band size: 70 kDa
        Observed band size: 70 kDa



        Western blot analysis on Jurkat Cell lysate.

      References

      This product has been referenced in:

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