Anti-ZAP70 antibody [SPM362] (ab233985)

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Overview

  • Product name

    Anti-ZAP70 antibody [SPM362]
    See all ZAP70 primary antibodies

  • Description

    Mouse monoclonal [SPM362] to ZAP70

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment corresponding to Human ZAP70 aa 1-254. Encompassing SH2 domains of human ZAP70.
    Sequence:

    MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSL VHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRK PCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVE KLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYAL SLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCL KEAC


    Database link: P43403

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab233985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Function

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

  • Tissue specificity

    Expressed in T- and natural killer cells.

  • Involvement in disease

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.

  • Domain

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

  • Cellular localization

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

  • Database links

    • Alternative names

      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC 2.7.10.2 antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain of T cell receptor associated protein kinase 70 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 antibody [SPM362] (ab233985)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 antibody [SPM362] (ab233985)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for ZAP70 using ab233985 at 1 μg/ml in immunohistochemical analysis.

    References

    ab233985 has not yet been referenced specifically in any publications.

    Publishing research using ab233985? Please let us know so that we can cite the reference in this datasheet.

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