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RecombinantRabMAb

Recombinant Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

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Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
  • Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
  • Flow Cytometry (Intracellular) - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
  • Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), Dot blot, WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 HRP Unconjugated

Overview

  • Product name

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free
    See all ZAP70 primary antibodies

  • Description

    Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), Dot blot, WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247483 is the carrier-free version of ab76501.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
ICC/IF
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

  • Tissue specificity

    Expressed in T- and natural killer cells.

  • Involvement in disease

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.

  • Domain

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

  • Cellular localization

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

  • Database links

    • Alternative names

      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC 2.7.10.2 antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain of T cell receptor associated protein kinase 70 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    Images

    • Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/5000 dilution

      Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysate
      Lane 2 : Jurkat (human acute T cell leukemia) treated with pervanadate whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 70 kDa
      Observed band size: 70 kDa


      Exposure time: 3 minutes


      This data was developed using ab76501, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      This data was developed using ab76501, the same antibody clone in a different buffer formulation.Dot blot analysis of varying amounts of ZAP70 (pY292) phospho peptide (Lane 1) and ZAP70 non-phospho peptide (Lane 2) using ab76501 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).Exposure time: 3 minutes.
    • Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      This data was developed using ab76501, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of untreated and pervanadate treated Jurkat (human acute T cell leukemia) cells labelling ZAP70 (phospho Y292) with ab76501 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Treated samples were starved overnight followed by incubation with 1mM pervanadate for 30 minutes. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Negative control using secondary antibody only.Image shows increased cytoplasmic staining after pervanadate treatment.
    • Flow Cytometry (Intracellular) - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      Flow Cytometry (Intracellular) - Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

      This data was developed using ab76501, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).

       

    • Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)
      Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab247483? Please let us know so that we can cite the reference in this datasheet.

    ab247483 has not yet been referenced specifically in any publications.

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