Recombinant
RabMAb

Recombinant Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free (ab247483)

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Overview

  • Product name

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] - BSA and Azide free
    See all ZAP70 primary antibodies

  • Description

    Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects ZAP70 when phosphorylated at Tyrosine 292.

  • Tested applications

    Suitable for: Dot blot, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ZAP70 (phospho Y292). The exact sequence is proprietary.

  • General notes

    ab247483 is the carrier-free version of ab76501 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247483 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab247483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

    • Tissue specificity

      Expressed in T- and natural killer cells.

    • Involvement in disease

      Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.

    • Domain

      The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

    • Post-translational
      modifications

      Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

    • Cellular localization

      Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
    • Information by UniProt

    • Database links

      • Alternative names

        • 70 kDa zeta associated protein antibody
        • 70 kDa zeta-associated protein antibody
        • EC 2.7.10.2 antibody
        • FLJ17670 antibody
        • FLJ17679 antibody
        • Selective T cell defect antibody
        • SRK antibody
        • STD antibody
        • Syk related tyrosine kinase antibody
        • Syk-related tyrosine kinase antibody
        • Truncated ZAP kinase antibody
        • Tyrosine protein kinase ZAP70 antibody
        • Tyrosine-protein kinase ZAP-70 antibody
        • TZK antibody
        • ZAP 70 antibody
        • ZAP70 antibody
        • ZAP70_HUMAN antibody
        • Zeta chain associated protein kinase 70kD antibody
        • Zeta chain associated protein kinase 70kDa antibody
        • Zeta chain associated protein kinase 70kDa isoform 1 antibody
        • Zeta chain associated protein kinase 70kDa isoform 2 antibody
        • Zeta chain of T cell receptor associated protein kinase 70 antibody
        • Zeta chain TCR associated protein kinase 70kD antibody
        • Zeta chain TCR associated protein kinase 70kDa antibody
        see all

      References

      ab247483 has not yet been referenced specifically in any publications.

      Publishing research using ab247483? Please let us know so that we can cite the reference in this datasheet.

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