Recombinant
RabMAb

Recombinant Anti-ZAP70 (phospho Y292) antibody [EPR1073] (HRP) (ab199245)

Overview

  • Product name

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] (HRP)
    See all ZAP70 primary antibodies
  • Description

    Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZAP70 (phospho Y292). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat whole cell lysate treated with pervanadate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

Target

  • Function

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • Tissue specificity

    Expressed in T- and natural killer cells.
  • Involvement in disease

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • Domain

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • Cellular localization

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70 kDa zeta associated protein antibody
    • 70 kDa zeta-associated protein antibody
    • EC 2.7.10.2 antibody
    • FLJ17670 antibody
    • FLJ17679 antibody
    • Selective T cell defect antibody
    • SRK antibody
    • STD antibody
    • Syk related tyrosine kinase antibody
    • Syk-related tyrosine kinase antibody
    • Truncated ZAP kinase antibody
    • Tyrosine protein kinase ZAP70 antibody
    • Tyrosine-protein kinase ZAP-70 antibody
    • TZK antibody
    • ZAP 70 antibody
    • ZAP70 antibody
    • ZAP70_HUMAN antibody
    • Zeta chain associated protein kinase 70kD antibody
    • Zeta chain associated protein kinase 70kDa antibody
    • Zeta chain associated protein kinase 70kDa isoform 1 antibody
    • Zeta chain associated protein kinase 70kDa isoform 2 antibody
    • Zeta chain of T cell receptor associated protein kinase 70 antibody
    • Zeta chain TCR associated protein kinase 70kD antibody
    • Zeta chain TCR associated protein kinase 70kDa antibody
    see all

Images

  • All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (HRP) (ab199245) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate treated with Pervanadate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate treated with Pervanadate, the membrane was then incubated with Alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 15 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before being incubated with ab199245 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab199245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199245.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up