Product nameAnti-ZAP70 (phospho Y319) antibody
See all ZAP70 primary antibodies
DescriptionRabbit polyclonal to ZAP70 (phospho Y319)
Tested applicationsSuitable for: IP, Flow Cytmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human ZAP70 (phospho Y319). The exact sequence is proprietary. NP_001070.2
Database link: P43403
- IP: Jurkat (treated with hydrogen peroxide) whole cell lysate. Flow cyt: Jurkat (treated with hydrogen peroxide) cells.
This antibody has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264260 in the following tested applications.
|IP||Use a concentration of 2 - 5 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
in 150 μL volume
FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificityExpressed in T- and natural killer cells.
Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
modificationsPhosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
- Information by UniProt
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 22.214.171.124 antibody
1.5% PFA-fixed, 90% methonol permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (treated with hydrogen peroxide for 3 minutes) cells labeling ZAP70 (phospho Y319) with ab264260 at 1 μg / 106 cells in 150 μL (blue) compared to an isotype anti-KLH control in hydrogen peroxide-treated cells (black) and ab264260 in untreated cells (red).
ZAP70 (phospho Y319) was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) (treated with hydrogen peroxide)whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264260 at 3 μg/mg lysate. Western blot was performed on the immunoprecipitates using an anti-ZAP70 antibody ab245411 at 1 μg/ml.
Lane 1: ab264260 IP in Jurkat (treated) whole cell lysate.
Lane 2: Control IgG IP in Jurkat (treated) whole cell lysate.
Exposure time: 3 minutes.
ab264260 has not yet been referenced specifically in any publications.