Key features and details
- Rabbit monoclonal [EP2291Y] to ZAP70 (phospho Y492)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZAP70 (phospho Y492) antibody [EP2291Y]
See all ZAP70 primary antibodies
DescriptionRabbit monoclonal [EP2291Y] to ZAP70 (phospho Y492)
Specificityab76306 only detects ZAP70 phosphorylated on Tyrosine 492.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
A synthetic phospho-peptide corresponding to residues surrounding Tyr492 of human ZAP70
- Jurkat cell lysates, untreated or treated with pervanadate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificityExpressed in T- and natural killer cells.
Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
modificationsPhosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
- Information by UniProt
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 184.108.40.206 antibody
All lanes : Anti-ZAP70 (phospho Y492) antibody [EP2291Y] (ab76306) at 1/20000 dilution
Lane 1 : Jurkat cell lysates, untreated
Lane 2 : Jurkat cell lysates, treated with pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
ab76306 has been referenced in 1 publication.
- Chiang YJ et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-Cell adhesion and migration. PLoS One 8:e61761 (2013). WB . PubMed: 23620790