Key features and details
- Rabbit polyclonal to ZAP70 (phospho Y493)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ZAP70 (phospho Y493) antibody
See all ZAP70 primary antibodies
DescriptionRabbit polyclonal to ZAP70 (phospho Y493)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human ZAP70 (phospho Y493). The exact sequence is proprietary.
Database link: P43403
- WB: PMA/TPA, ATP or Anisomycin-treated Jurkat cell lysates; IHC-P: Human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/2000 - 1/5000. Predicted molecular weight: 70 kDa.|
FunctionPlays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Tissue specificityExpressed in T- and natural killer cells.
Involvement in diseaseDefects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains.
DomainThe SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
modificationsPhosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
Cellular localizationCytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
- Information by UniProt
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 220.127.116.11 antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, labeling ZAP70 (phospho Y493) with ab194800 at 1/50 dilution.
All lanes : Anti-ZAP70 (phospho Y493) antibody (ab194800) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : PMA/TPA-treated Jurkat cell lysate
Lane 3 : Anisomycin-treated Jurkat cell lysate
Lane 4 : ATP-treated Jurkat cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 30 seconds
Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes, treated by Anisomycin (5 ug/ml) at 37°C for 30 minutes after serum-starvation overnight, or treated by ATP(5 mM) at 30°C for 1 hour.
Blocking buffer: 3% BSA.
ab194800 has been referenced in 1 publication.
- Liang W et al. Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation. Front Immunol 9:78 (2018). PubMed: 29434598