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    zbed3-antibody-ab106383.pdf

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Stem Cells Signaling Pathways Wnt Cytoplasmic
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Anti-ZBED3 antibody (ab106383)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-ZBED3 antibody (ab106383)

    Key features and details

    • Rabbit polyclonal to ZBED3
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-ZBED3 antibody
    • Description

      Rabbit polyclonal to ZBED3
    • Host species

      Rabbit
    • Specificity

      ab106383 is predicted to have no cross-reactivity to ZBED1 or ZBED2.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      17 amino acid synthetic peptide from near the N-terminus of Human ZBED3 (NP_115743).

    • Positive control

      • Rat lung tissue lysate

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at 4°C (stable for up to 12 months).
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Cytoplasmic
      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Nuclear

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab106383 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.
    Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa.

    Target

    • Sequence similarities

      Contains 1 BED-type zinc finger.
    • Target information above from: UniProt accession Q96IU2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 84327 Human
      • Entrez Gene: 72114 Mouse
      • Entrez Gene: 361881 Rat
      • SwissProt: Q96IU2 Human
      • Unigene: 584988 Human
      • Unigene: 3774 Mouse
      • Unigene: 103511 Rat
      • Alternative names

        • MGC15435 antibody
        • ZBED3 antibody
        • ZBED3_HUMAN antibody
        • Zinc finger BED domain containing 3 antibody
        • Zinc finger BED domain-containing protein 3 antibody
        • Zinc finger BED type containing 3 antibody
        see all

      Images

      • Western blot - Anti-ZBED3 antibody (ab106383)
        Western blot - Anti-ZBED3 antibody (ab106383)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab106383? Please let us know so that we can cite the reference in this datasheet.

      ab106383 has been referenced in 1 publication.

      • Shi X  et al. Zbed3 promotes proliferation and invasion of lung cancer partly through regulating the function of Axin-Gsk3ß complex. J Cell Mol Med 23:1014-1021 (2019). PubMed: 30417576

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-ZBED3 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      50 µg
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Sample
      Human Cell lysate - whole cell (HeLa, RPE-1)
      Specification
      HeLa, RPE-1
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Sep 20 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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