Anti-ZBT10 antibody (ab117786)
Key features and details
- Rabbit polyclonal to ZBT10
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZBT10 antibody
See all ZBT10 primary antibodies -
Description
Rabbit polyclonal to ZBT10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide, corresponding to a region within amino acids 50-100 of Human ZBT10 (NP_076418.3).
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Positive control
- Jurkat and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab117786 was affinity purified using an epitope specific to ZBT10 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rabbit
Cow
Pig
Chimpanzee
Gorilla
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 95 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Function
May be involved in transcriptional regulation. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 65986 Human
- SwissProt: Q96DT7 Human
- Unigene: 591868 Human
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Alternative names
- FLJ12752 antibody
- OTTHUMP00000207119 antibody
- RINZF antibody
see all
Images
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Western blot - Anti-ZBT10 antibody (ab117786)All lanes : Anti-ZBT10 antibody (ab117786) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : Jurkat whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ZBT10 antibody (ab117786)Detection of ZBT10 by Western Blot of Immunprecipitate.
ab117786 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117786 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
References (0)
ab117786 has not yet been referenced specifically in any publications.
