Overview

  • Product name
    Anti-ZBT10 antibody
    See all ZBT10 primary antibodies
  • Description
    Rabbit polyclonal to ZBT10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Pig, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 50-100 of Human ZBT10 (NP_076418.3).

  • Positive control
    • Jurkat and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117786 was affinity purified using an epitope specific to ZBT10 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 2 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • FLJ12752 antibody
      • OTTHUMP00000207119 antibody
      • RINZF antibody
      • RINZFC antibody
      • ZBT10_HUMAN antibody
      • ZBTB10 antibody
      • Zinc finger and BTB domain containing protein 10 antibody
      • Zinc finger and BTB domain-containing protein 10 antibody
      • Zinc finger protein RIN ZF antibody
      see all

    Images

    • Western blot - Anti-ZBT10 antibody (ab117786)
      Western blot - Anti-ZBT10 antibody (ab117786)
      All lanes : Anti-ZBT10 antibody (ab117786) at 0.1 µg/ml

      Lane 1 : 293T whole cell lysates at 50 µg
      Lane 2 : 293T whole cell lysates at 15 µg
      Lane 3 : Jurkat whole cell lysates at 50 µg

      Developed using the ECL technique.

      Predicted band size: 95 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-ZBT10 antibody (ab117786)
      Immunoprecipitation - Anti-ZBT10 antibody (ab117786)
      Detection of ZBT10 by Western Blot of Immunprecipitate.
      ab117786 at 1µg/ml staining ZBT10 in 293T whole cell lysate immunoprecipitated using ab117786 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab117786 has not yet been referenced specifically in any publications.

    Publishing research using ab117786? Please let us know so that we can cite the reference in this datasheet.

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