Anti-ZBT10 antibody (ab80879)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ZBT10 antibody
See all ZBT10 primary antibodies -
Description
Rabbit polyclonal to ZBT10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human ZBT10 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab90846) -
Positive control
- This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HEK293; HepG2; HUVEC; SHSY-5Y.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab80879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). |
Target
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Function
May be involved in transcriptional regulation. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 65986 Human
- Entrez Gene: 80338 Rat
- SwissProt: Q96DT7 Human
- SwissProt: Q9WTY8 Rat
- Unigene: 591868 Human
- Unigene: 162568 Rat
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Alternative names
- FLJ12752 antibody
- OTTHUMP00000207119 antibody
- RINZF antibody
see all
Images
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Western blot - Anti-ZBT10 antibody (ab80879)All lanes : Anti-ZBT10 antibody (ab80879) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 67 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab80879 detects a strong band at 100 kDa which is thought to be the target protein as well as a band at 67 kDa. It is thought that the bands around 67 kDa corresponds to isoform 2 which has a predicted molecular weight of 65 kDa (SwissProt data).
Protocols
Datasheets and documents
References
ab80879 has not yet been referenced specifically in any publications.
