Anti-ZBT10 antibody (ab80879)

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Western blot - Anti-ZBT10 antibody (ab80879)

    Key features and details

    • Rabbit polyclonal to ZBT10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-ZBT10 antibody
      See all ZBT10 primary antibodies

    • Description

      Rabbit polyclonal to ZBT10

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human ZBT10 aa 300-400 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab90846)

    • Positive control

      • This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HEK293; HepG2; HUVEC; SHSY-5Y.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab80879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rat
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).

    Target

    • Function

      May be involved in transcriptional regulation.

    • Sequence similarities

      Contains 1 BTB (POZ) domain.
      Contains 2 C2H2-type zinc fingers.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • FLJ12752 antibody
        • OTTHUMP00000207119 antibody
        • RINZF antibody
        • RINZFC antibody
        • ZBT10_HUMAN antibody
        • ZBTB10 antibody
        • Zinc finger and BTB domain containing protein 10 antibody
        • Zinc finger and BTB domain-containing protein 10 antibody
        • Zinc finger protein RIN ZF antibody
        see all

      Images

      • Western blot - Anti-ZBT10 antibody (ab80879)
        Western blot - Anti-ZBT10 antibody (ab80879)
        All lanes : Anti-ZBT10 antibody (ab80879) at 1 µg/ml

        Lane 1 : Human liver tissue lysate - total protein (ab29889)
        Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
        Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
        Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
        Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
        Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 92 kDa
        Observed band size: 100 kDa why is the actual band size different from the predicted?
        Additional bands at: 46 kDa, 67 kDa (possible isoform). We are unsure as to the identity of these extra bands.


        Exposure time: 20 minutes


        ab80879 detects a strong band at 100 kDa which is thought to be the target protein as well as a band at 67 kDa. It is thought that the bands around 67 kDa corresponds to isoform 2 which has a predicted molecular weight of 65 kDa (SwissProt data).

      References (0)

      Publishing research using ab80879? Please let us know so that we can cite the reference in this datasheet.

      ab80879 has not yet been referenced specifically in any publications.

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