Product nameAnti-ZBT10 antibody
See all ZBT10 primary antibodies
DescriptionRabbit polyclonal to ZBT10
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human ZBT10 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HEK293; HepG2; HUVEC; SHSY-5Y.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 2 C2H2-type zinc fingers.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- FLJ12752 antibody
- OTTHUMP00000207119 antibody
- RINZF antibody
All lanes : Anti-ZBT10 antibody (ab80879) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 67 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab80879 detects a strong band at 100 kDa which is thought to be the target protein as well as a band at 67 kDa. It is thought that the bands around 67 kDa corresponds to isoform 2 which has a predicted molecular weight of 65 kDa (SwissProt data).
ab80879 has not yet been referenced specifically in any publications.