Product nameAnti-ZBTB2 antibody
DescriptionRabbit polyclonal to ZBTB2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide, corresponding to a region within amino acids 464-514 of Human ZBTB2 (NP_065912.1).
- 293T, Jurkat, HeLa and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117756 was affinity purified using an epitope specific to ZBTB2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab117756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be involved in transcriptional regulation.
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- bA351K16.2 antibody
- Gm1103 antibody
- KIAA1483 antibody
All lanes : Anti-ZBTB2 antibody (ab117756) at 0.04 µg/ml
Lane 1 : 293T cell line lysate at 50 µg
Lane 2 : 293T cell line lysate at 15 µg
Lane 3 : Jurkat cell line lysate at 50 µg
Lane 4 : HeLa cell line lysate at 50 µg
Lane 5 : NIH3T3 cell line lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds
Detection of ZBTB2 by Western Blot of Immunprecipitate.
ab117756 at 1µg/ml staining ZBTB2 in 293T whole cell lysate immunoprecipitated using ab117756 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab117756 has not yet been referenced specifically in any publications.