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Rabbit polyclonal to ZBTB2
Predicted to work with:
Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide, corresponding to a region within amino acids 464-514 of Human ZBTB2 (NP_065912.1).
293T, Jurkat, HeLa and NIH3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
ab117756 was affinity purified using an epitope specific to ZBTB2 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in transcriptional regulation.
Contains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - Anti-ZBTB2 antibody (ab117756)
All lanes : Anti-ZBTB2 antibody (ab117756) at 0.04 µg/ml Lane 1 : 293T cell line lysate at 50 µg Lane 2 : 293T cell line lysate at 15 µg Lane 3 : Jurkat cell line lysate at 50 µg Lane 4 : HeLa cell line lysate at 50 µg Lane 5 : NIH3T3 cell line lysate at 50 µg Developed using the ECL technique. Predicted band size: 57 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-ZBTB2 antibody (ab117756)
Detection of ZBTB2 by Western Blot of Immunprecipitate.
ab117756 at 1µg/ml staining ZBTB2 in 293T whole cell lysate immunoprecipitated using ab117756 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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