Overview

  • Product name
  • Description
    Rabbit polyclonal to ZBTB38
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human ZBTB38 (NP_001073881.2 (GeneID 253461)).

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.50
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 134 kDa).
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Acts as a transcriptional activator. May be involved in the differentiation and/or survival of late postmitotic neurons.
  • Sequence similarities
    Contains 1 BTB (POZ) domain.
    Contains 10 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIBZ antibody
    • FLJ22332 antibody
    • FLJ31131 antibody
    • FLJ35036 antibody
    • ZBT38_HUMAN antibody
    • ZBTB38 antibody
    • Ziinc finger and BTB domain containing protein 38 antibody
    • Zinc finger and BTB domain-containing protein 38 antibody
    see all

Images

  • All lanes : Anti-ZBTB38 antibody (ab112051) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 134 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  •  

     

  • Lane 1 : Anti-ZBTB38 antibody (ab112051) at 1 µg/ml (lysate)
    1 mg lysate/IP; 20% of IP loaded/lane)
    Lane 2 : Recombinant Human NT-4 protein (denatured) (ab113151) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (6 µg ab112051/mg
    Lane 2 : Control IgG

    Predicted band size: 134 kDa

References

ab112051 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your call today and for your questions about ab112051.

Sincethis antibody has not been tested inChIP-Seq, I can offer a discount off a future purchase if you buy ab112051 now, test it in ChIP-Seqand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount wouldworth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab112051 in ChIP-Seq. I will then send a discount code.

2. Purchase ab112051 either by phone, fax, or online (www.abcam.com).

3. Test it in ChIP-Seq.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab112051 turns out to be unsuitable for ChIP-Seq, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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