Key features and details
- Mouse monoclonal [PCRP-ZMYM3-2F10] to ZBTB39
- Suitable for: Protein Array, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-ZBTB39 antibody [PCRP-ZMYM3-2F10]
See all ZBTB39 primary antibodies
DescriptionMouse monoclonal [PCRP-ZMYM3-2F10] to ZBTB39
Tested applicationsSuitable for: Protein Array, Flow Cyt, ICCmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human ZBTB39.
Database link: O15060
- Flow cyt: HeLa cells. ICC: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99.9% PBS
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate
Our Abpromise guarantee covers the use of ab277109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells.|
|ICC||Use a concentration of 1 - 2 µg/ml.|
- Information by UniProt
- KIAA0352 antibody
- ZBT39_HUMAN antibody
- ZBTB39 antibody
Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ZBTB39 with ab277109 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ZBTB39 using ab277109 at 2 µg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277109.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab277109 has not yet been referenced specifically in any publications.