Anti-ZBTB48 antibody - N-terminal (ab225935)
Key features and details
- Rabbit polyclonal to ZBTB48 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZBTB48 antibody - N-terminal -
Description
Rabbit polyclonal to ZBTB48 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human ZBTB48 aa 1-300 (N terminal).
Database link: P10074 -
Positive control
- WB: HEK-293T, K562, HepG2 and U-251 MG whole cell lysates; Mouse liver tissue lysate. IHC-P: Human spleen and lung tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
Target
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Function
Binds to and regulates the J and/or S elements in MHC II promoter. -
Tissue specificity
Detected in adrenal gland and neuroblastoma. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 11 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3104 Human
- Entrez Gene: 100090 Mouse
- Omim: 165270 Human
- SwissProt: P10074 Human
- SwissProt: Q1H9T6 Mouse
- Unigene: 502330 Human
- Unigene: 24661 Mouse
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Alternative names
- 0610011D15Rik antibody
- AI327031 antibody
- GLI Kruppel family member HKR3 antibody
see all
Images
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All lanes : Anti-ZBTB48 antibody - N-terminal (ab225935) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5 : Mouse liver tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa -
Paraffin-embedded human spleen tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225935 has not yet been referenced specifically in any publications.