Key features and details
- Rabbit polyclonal to ZBTB48 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ZBTB48 antibody - N-terminal
DescriptionRabbit polyclonal to ZBTB48 - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human ZBTB48 aa 1-280 (N terminal).
MDGSFVQHSVRVLQELNKQREKGQYCDATLDVGGLVFKAHWSVLACCSHF FQSLYGDGSGGSVVLPAGFAEIFGLLLDFFYTGHLALTSGNRDQVLLAAR ELRVPEAVELCQSFKPKTSVGQAAGGQSGLGPPASQNVNSHVKEPAGLEE EEVSRTLGLVPRDQEPRGSHSPQRPQLHSPAQSEGPSSLCGKLKQALKPC PLEDKKPEDCKVPPRPLEAEGAQLQGGSNEWEVVVQVEDDGDGDYMSEPE AVLTRRKSNVIRKPCAAEPALSAGSLAAEP
Database link: P10074
- WB: HEK-293T, K562, HepG2 and U-251 MG whole cell lysates; Mouse liver tissue lysate. IHC-P: Human spleen and lung tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
FunctionBinds to and regulates the J and/or S elements in MHC II promoter.
Tissue specificityDetected in adrenal gland and neuroblastoma.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 11 C2H2-type zinc fingers.
- Information by UniProt
- 0610011D15Rik antibody
- AI327031 antibody
- GLI Kruppel family member HKR3 antibody
All lanes : Anti-ZBTB48 antibody - N-terminal (ab225935) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5 : Mouse liver tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Paraffin-embedded human spleen tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung tissue stained for ZBTB48 using ab225935 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225935 has not yet been referenced specifically in any publications.