Recombinant
RabMAb

Anti-ZBTB7A antibody [EPR13178(B)] (ab175918)

Overview

  • Product name
    Anti-ZBTB7A antibody [EPR13178(B)]
    See all ZBTB7A primary antibodies
  • Description
    Rabbit monoclonal [EPR13178(B)] to ZBTB7A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: O95365

  • Positive control
    • MCF-7, HepG2, K562, Hela cell lysates, human bladder carcinoma tissue, human tonsil tissue, MCF7 and Hela cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13178(B)
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab175918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250.

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
    • Tissue specificity
      Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level).
    • Sequence similarities
      Contains 1 BTB (POZ) domain.
      Contains 4 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp547O146 antibody
      • Factor binding IST protein 1 antibody
      • Factor that binds to inducer of short transcripts protein 1 antibody
      • FBI-1 antibody
      • FBI1 antibody
      • HIV-1 1st-binding protein 1 antibody
      • HIV-1 inducer of short transcripts binding protein antibody
      • HIV-1 inducer of short transcripts-binding factor 1 antibody
      • Leukemia/lymphoma-related factor antibody
      • LRF antibody
      • lymphoma related factor antibody
      • MGC99631 antibody
      • POK erythroid myeloid ontogenic factor antibody
      • Pokemon antibody
      • POZ and Krueppel erythroid myeloid ontogenic factor antibody
      • TIP21 antibody
      • TTF-I-interacting peptide 21 antibody
      • ZBT7A_HUMAN antibody
      • ZBTB7 antibody
      • ZBTB7A antibody
      • Zinc finger and BTB domain containing 7A antibody
      • zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein antibody
      • Zinc finger and BTB domain-containing protein 7A antibody
      • Zinc finger protein 857A antibody
      • Zinc finger- and BTB domain-containing protein 7 antibody
      • ZNF857A antibody
      see all

    Images

    • All lanes : Anti-ZBTB7A antibody [EPR13178(B)] (ab175918) at 1/10000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Hela cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 61 kDa

    • Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution 

    • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution

    • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.

    References

    This product has been referenced in:
    • Shen Y  et al. Negative feedback loop between ZBTB7A and TGF-ß in breast cancer. Oncol Lett 14:1403-1410 (2017). Read more (PubMed: 28789356) »
    • de Vasconcellos JF  et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. Proc Natl Acad Sci U S A 114:E5664-E5672 (2017). WB ; Human . Read more (PubMed: 28652347) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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