Product nameAnti-ZBTB7A antibody [EPR13178(B)]
See all ZBTB7A primary antibodies
DescriptionRabbit monoclonal [EPR13178(B)] to ZBTB7A
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human ZBTB7A aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: O95365
- MCF-7, HepG2, K562, Hela cell lysates, human bladder carcinoma tissue, human tonsil tissue, MCF7 and Hela cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
For unpurified use at 1/100 - 1/250.
|IP||1/10 - 1/100.|
FunctionPlays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
Tissue specificityWidely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level).
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers.
- Information by UniProt
- DKFZp547O146 antibody
- Factor binding IST protein 1 antibody
- Factor that binds to inducer of short transcripts protein 1 antibody
All lanes : Anti-ZBTB7A antibody [EPR13178(B)] (ab175918) at 1/10000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling ZBTB7A with purified ab175918 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunofluorescent staining of HeLa cells using labeling ZBTB7A with ab175918 at 1/100 dilution
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ZBTB7A with ab175918 at 1/100 dilution
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling ZBTB7A with ab175918 at 1/100 dilution
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using labeling ZBTB7A with ab175918 at 1/10 dilution.
This product has been referenced in:
- Shen Y et al. Negative feedback loop between ZBTB7A and TGF-ß in breast cancer. Oncol Lett 14:1403-1410 (2017). Read more (PubMed: 28789356) »
- de Vasconcellos JF et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. Proc Natl Acad Sci U S A 114:E5664-E5672 (2017). WB ; Human . Read more (PubMed: 28652347) »