Key features and details
- Rabbit polyclonal to ZC3H11A
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZC3H11A antibody
See all ZC3H11A primary antibodies
DescriptionRabbit polyclonal to ZC3H11A
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human ZC3H11A aa 700-750. The exact sequence is proprietary. (NP_055642.3)
Database link: O75152
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to ZC3H11A immobilized on solid support.
Our Abpromise guarantee covers the use of ab241612 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 89 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceZC3H11A, also known as zinc finger CCCH type containing 11A, contains 3 C3H1-type zinc fingers.
- ZC3H11A antibody
- DKFZp686D03108 antibody
- DKFZp686F14109 antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ZC3H11A using ab241612 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
ZC3H11A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241612 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241612 at 0.1 µg/ml.
Lane 1: ab241612 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes : Anti-ZC3H11A antibody (ab241612) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 3 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241612 has not yet been referenced specifically in any publications.