Overview

  • Product name

  • Description

    Rabbit polyclonal to ZC3H11A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human ZC3H11A aa 700-750. The exact sequence is proprietary. (NP_055642.3)
    Database link: O75152

  • Positive control

    • WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to ZC3H11A immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 89 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    ZC3H11A, also known as zinc finger CCCH type containing 11A, contains 3 C3H1-type zinc fingers.
  • Database links

  • Alternative names

    • ZC3H11A antibody
    • DKFZp686D03108 antibody
    • DKFZp686F14109 antibody
    • DKFZp781G2455 antibody
    • KIAA0663 antibody
    • ZC3HDC11A antibody
    • Zinc finger CCCH domain containing protein 11A antibody
    • zinc finger CCCH type containing 11A antibody
    • Zinc finger CCCH type domain containing 11A antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ZC3H11A using ab241612 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

  • ZC3H11A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241612 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241612 at 0.1 µg/ml.

    Lane 1: ab241612 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 seconds.

  • All lanes : Anti-ZC3H11A antibody (ab241612) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 89 kDa


    Exposure time: 3 seconds

References

ab241612 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241612.
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