Product nameAnti-ZC3H11A antibody
See all ZC3H11A primary antibodies
DescriptionRabbit polyclonal to ZC3H11A
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human ZC3H11A aa 700-750. The exact sequence is proprietary. (NP_055642.3)
Database link: O75152
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to ZC3H11A immobilized on solid support.
Our Abpromise guarantee covers the use of ab241612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 89 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceZC3H11A, also known as zinc finger CCCH type containing 11A, contains 3 C3H1-type zinc fingers.
- ZC3H11A antibody
- DKFZp686D03108 antibody
- DKFZp686F14109 antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for ZC3H11A using ab241612 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
ZC3H11A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241612 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241612 at 0.1 µg/ml.
Lane 1: ab241612 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes : Anti-ZC3H11A antibody (ab241612) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 3 seconds
ab241612 has not yet been referenced specifically in any publications.