Overview

  • Product name

  • Description

    Rabbit polyclonal to ZC3H11A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 332 - 585 of Human ZC3H11A (BC014268).

  • Positive control

    • Human fetal brain, lung and kidney lysate. Human breast carcinoma tissue IF/ICC: MCF7 cell line.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 89 kDa.
ELISA 1/20000 - 1/80000.
IHC-P 1/100 - 1/500.

Target

  • Relevance

    ZC3H11A, also known as zinc finger CCCH type containing 11A, contains 3 C3H1-type zinc fingers.
  • Database links

  • Alternative names

    • ZC3H11A antibody
    • DKFZp686D03108 antibody
    • DKFZp686F14109 antibody
    • DKFZp781G2455 antibody
    • KIAA0663 antibody
    • ZC3HDC11A antibody
    • Zinc finger CCCH domain containing protein 11A antibody
    • zinc finger CCCH type containing 11A antibody
    • Zinc finger CCCH type domain containing 11A antibody
    see all

Images

  • ab99930 stained MCF7 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99930 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-ZC3H11A antibody (ab99930) at 1/500 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : Human fetal kidney lysate

    Predicted band size: 89 kDa

  • ab99930, at 1/100 dilution, staining ZC3H11A in formalin-fixed paraffin-embedded Human breast carcinoma tissue.

References

ab99930 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

DISCOUNT CODE: XXXXXXXXXX
Expiration date: 07-09-2012

I am very pleased to hear you would like to use ab99930 inMouse and send us an image of your results.

This code will give you 1 free [PRIMARY ANTIBODY/PROTEIN] before the expiration date. To redeem this offer, please submit an Abreview with the image for [Mouse] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

PS: Pleaseorder the product online on Abcam website.

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Answer

Thank you for contacting us.

The predicted species reference is based on the sequence homology between human and mouse only. We currently do not hold any data to show this antibody will work in mouse however based on immunogen sequence homology, it can work.

If you are interested in testing this antibody in mouse then please click the below link for more information about eh testing discount we can give

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I can also offer an image discount; for this discount you just need to submit an mouse species related Abreview. In case the antibody does not work, we will refund your money.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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