Key features and details
- Rabbit polyclonal to ZC3H12D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZC3H12D antibody
DescriptionRabbit polyclonal to ZC3H12D
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
A 16 amino acid peptide from the intermediate residues of Human ZC3H12D protein (NP_997243).
- WB: Fetal small intestine lysate and fetal spleen lystae IHC-P: Fetal small intestine
FormLyophilized:Add 200 ul of sterile distilled water
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab100862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 58 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay regulate cell growth likely by suppressing RB1 phosphorylation (PubMed:19531561). May function as RNase and regulate the levels of target RNA species (Potential). In association with ZC3H12A enhances the degradation of interleukin IL-6 mRNA level in activated macrophages (PubMed:26134560). Serve as a tumor suppressor in certain leukemia cells (PubMed:17210687). Overexpression inhibits the G1 to S phase progression through suppression of RB1 phosphorylation (PubMed:19531561).
Tissue specificityExpressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.
Involvement in diseaseA chromosomal aberration involving ZC3H12D may be the cause of the transformation of follicular lymphoma (FL) to diffuse large B-cell lymphoma (DLBCL). Translocation t(2;6)(p12;q25) with IGK. Resulting protein may not be expressed.
Sequence similaritiesBelongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger.
Cellular localizationCytoplasm. Nucleus; Cytoplasm. Localized as discrete granules. Colocalized with mRNA-processing body markers, AGO2 and DCP1A, but not with a stress granule maker, TIA1, in the cytoplasm and Cytoplasm, P-body. Colocalizes with ZC3H12A in GW bodies (GWBs) (PubMed:26134560).
- Information by UniProt
- C6orf95 antibody
- ZC3H12D antibody
- FLJ00361 antibody
All lanes : Anti-ZC3H12D antibody (ab100862) at 1/500 dilution
Lane 1 : Human fetal small intestine lysate
Lane 2 : Human fetal spleen lysate
Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
ab100862, at a 1/100 dilution, staining ZC3H12D in formalin-fixed, paraffin-embedded Human fetal small intestine tissue, showing cytoplasmic staining, by Immunohistochemistry.
ab100862 has been referenced in 1 publication.
- Huang S et al. The putative tumor suppressor Zc3h12d modulates toll-like receptor signaling in macrophages. Cell Signal : (2011). IHC-P ; Mouse . PubMed: 22036805