Overview

  • Product name

  • Description

    Rabbit polyclonal to ZC3H13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to the C terminal region of Human ZC3H13.

  • Positive control

    • HUVEC cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138150 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 197 kDa.
ICC/IF 1/200.

Use with paraformaldehyde fixed cells.

Target

  • Sequence similarities

    Contains 1 C3H1-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2600010B19Rik antibody
    • 3110050K21Rik antibody
    • 4930570G11Rik antibody
    • C87618 antibody
    • DKFZp434D1812 antibody
    • FLJ35669 antibody
    • KIAA0853 antibody
    • ZC3H13 antibody
    • ZC3HD_HUMAN antibody
    • Zinc finger CCCH domain-containing protein 13 antibody
    • zinc finger CCCH type containing 13 antibody
    see all

Images

  • ab138150 (1/200) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.05% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • Anti-ZC3H13 antibody (ab138150) at 1/500 dilution + HUVEC cell lysate at 30 µg

    Predicted band size: 197 kDa

References

ab138150 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted May 16 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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