Synthetic peptide within Human ZC3H4 aa 140-170 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9UPT8
Our Abpromise guarantee covers the use of ab174962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 140 kDa.|
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram) labeling ZC3H4 with ab174962 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human cervix carcinoma tissue labeling ZC3H4 with ab174962 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab174962 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"