Key features and details
- Rabbit polyclonal to ZC3H8
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-ZC3H8 antibody
See all ZC3H8 primary antibodies
DescriptionRabbit polyclonal to ZC3H8
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide within Human ZC3H8 aa 1-50. The exact sequence is proprietary. (NP_115883.2) .
Database link: Q8N5P1
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225624 was affinity purified using an epitope specific to ZC3H8 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
- Information by UniProt
- Fliz1 antibody
- Zc3h8 antibody
- ZC3H8_HUMAN antibody
All lanes : Anti-ZC3H8 antibody (ab225624) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 minutes
ZC3H8 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225624 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225624 at 1 µg/ml.
Lane 1: ab225624 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225624 has not yet been referenced specifically in any publications.