Product nameAnti-ZCCHC16 antibody
DescriptionRabbit polyclonal to ZCCHC16
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to amino acids 161-309 of Human ZCCHC16 (Q6ZR62).
- RT4, U-251 MG, Human Liver and Human Tonsil lysates; Human colon tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesContains 1 CCHC-type zinc finger.
- Information by UniProt
- Mammalian retrotransposon-derived protein 4 antibody
- Zcchc16 antibody
- ZCH16_HUMAN antibody
- Zinc finger CCHC domain-containing protein 16 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121184 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-ZCCHC16 antibody (ab121184) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121184 at 1/250 dilution staining ZCCHC16 in Paraffin-emebedded Human colon tissue by Immunohistochemistry.
ab121184 has not yet been referenced specifically in any publications.