Key features and details
- Rabbit polyclonal to ZCCHC4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZCCHC4 antibody
See all ZCCHC4 primary antibodies
DescriptionRabbit polyclonal to ZCCHC4
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment corresponding to a region within amino acids 2-246 of Human ZCCHC4 (UniProt ID: Q9H5U6).
- 293T, A431, Jurkat and Raji whole cell lysates; A431 cells; Human PC13 xenograft tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay be a methyltransferase.
Sequence similaritiesContains 1 CCHC-type zinc finger.
Contains 1 DHHC-type zinc finger.
- Information by UniProt
- 4930449I23Rik antibody
- DHHC domain containing zinc finger protein antibody
- HSPC052 antibody
Anti-ZCCHC4 antibody (ab154002) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS PAGE
Immunofluorescent analysis of paraformaldehyde fixed A431 cells labeling ZCCHC4 with ab154002 at 1/500 dilution. Lower image costained with Hoerchst 33342.
Immunohistochemical analysis of paraffin embedded Human PC13 xenograft tissue, labeling ZCCHC4 with ab154002 antibody at 1/500 dilution.
ab154002 has not yet been referenced specifically in any publications.