Overview

  • Product name

    Anti-ZCCHC4 antibody [EPR20492]
    See all ZCCHC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR20492] to ZCCHC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human ZCCHC4 aa 200-300 (Cysteine residue). The exact sequence is proprietary. (Co-immunization performed with both peptides, clone obtained by screening with peptide 2).
    Database link: Q9H5U6

    Synthetic peptide within Human ZCCHC4 aa 300-400 (Cysteine residue). The exact sequence is proprietary. (Co-immunization performed with both peptides, clone obtained by screening with peptide 2).
    Database link: Q9H5U6

  • Positive control

    • WB: Wild type HeLa whole cell lysate. Flow Cyt: HeLa and HepG2 cells. IP: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20492
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

This antibody works best on a semi-dry nitrocellulose membrane transfer system.

Flow Cyt 1/100.
IP 1/50.

Target

  • Function

    May be a methyltransferase.
  • Sequence similarities

    Contains 1 CCHC-type zinc finger.
    Contains 1 DHHC-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4930449I23Rik antibody
    • DHHC domain containing zinc finger protein antibody
    • HSPC052 antibody
    • MGC21108 antibody
    • OTTMUSP00000027189 antibody
    • zcchc4 antibody
    • ZCHC4_HUMAN antibody
    • ZGRF4 antibody
    • Zinc finger CCHC domain containing protein 4 antibody
    • Zinc finger CCHC domain-containing protein 4 antibody
    • Zinc finger CCHC type containing 4 antibody
    • Zinc finger GRF type containing 4 antibody
    • Zinc finger, CCHC domain containing 4 antibody
    see all

Images

  • All lanes : Anti-ZCCHC4 antibody [EPR20492] (ab211325) at 1/1000 dilution

    Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : ZCCHC4 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody works best on a semi-dry nitrocellulose membrane transfer system.

    These lysates were kindly provided by Prof Chuan He; University of Chicago.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling ZCCHC4 with ab211325 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • ZCCHC4 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab211325 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab211325 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). 

    Lane 2: ab211325 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211325 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This antibody works best on a semi-dry nitrocellulose membrane transfer system.

References

ab211325 has not yet been referenced specifically in any publications.

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