Product nameAnti-ZCCHC6 antibody
See all ZCCHC6 primary antibodies
DescriptionRabbit polyclonal to ZCCHC6
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide within Human ZCCHC6 aa 800-850. The exact sequence is proprietary.
Database link: Q5VYS8
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226294 was affinity purified using an epitope specific to ZCCHC6 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 171 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceZCCHC6 contains 3 CCHC-type zinc fingers.
- KIAA1711 antibody
- MGC38585 antibody
- PAP associated domain containing 6 antibody
All lanes : Anti-ZCCHC6 antibody (ab226294) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 3 minutes
Cells prepared using NETN lysis buffer.
ZCCHC6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226294 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226294 at 1 µg/ml.
Lane 1: ab226294 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab226294 has not yet been referenced specifically in any publications.