Overview

  • Product name

    Anti-ZCCHC8 antibody [EPR13612]
    See all ZCCHC8 primary antibodies
  • Description

    Rabbit monoclonal [EPR13612] to ZCCHC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ZCCHC8 aa 400-550. The exact sequence is proprietary.
    Database link: Q6NZY4

  • Positive control

    • Hela, 293 and K562 cell lysates, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13612
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
ICC/IF 1/100 - 1/250.

Target

  • Function

    May be involved in pre-mRNA splicing.
  • Sequence similarities

    Belongs to the ZCCHC8 family.
    Contains 1 CCHC-type zinc finger.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434E2220 antibody
    • ZCCHC8 antibody
    • ZCHC8_HUMAN antibody
    • Zinc finger CCHC domain containing 8 antibody
    • Zinc finger CCHC domain containing protein 8 antibody
    • Zinc finger CCHC domain-containing protein 8 antibody
    see all

Images

  • Immunofluorescence analysis of HeLa cells labeling ZCCHC8 with ab181152 at 1/250 dilution (left) and Dapi blue (right).  Fixative: -20℃ Acetone.  Secondary: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.

  • All lanes : Anti-ZCCHC8 antibody [EPR13612] (ab181152) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa



    Blocking buffer: 5% NFDM/TBST

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181152.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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