Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13612] to ZCCHC8
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-ZCCHC8 antibody [EPR13612]
See all ZCCHC8 primary antibodies
DescriptionRabbit monoclonal [EPR13612] to ZCCHC8
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ZCCHC8 aa 400-550. The exact sequence is proprietary.
Database link: Q6NZY4
- Hela, 293 and K562 cell lysates, HeLa cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 79 kDa.|
|ICC/IF||1/100 - 1/250.|
FunctionMay be involved in pre-mRNA splicing.
Sequence similaritiesBelongs to the ZCCHC8 family.
Contains 1 CCHC-type zinc finger.
- Information by UniProt
- DKFZp434E2220 antibody
- ZCCHC8 antibody
- ZCHC8_HUMAN antibody
Immunofluorescence analysis of HeLa cells labeling ZCCHC8 with ab181152 at 1/250 dilution (left) and Dapi blue (right). Fixative: -20℃ Acetone. Secondary: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.
All lanes : Anti-ZCCHC8 antibody [EPR13612] (ab181152) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 79 kDa
Blocking buffer: 5% NFDM/TBST
ab181152 has been referenced in 1 publication.
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244