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    zcchc9-antibody-ab122470.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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Anti-ZCCHC9 antibody (ab122470)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
  • Western blot - Anti-ZCCHC9 antibody (ab122470)

Key features and details

  • Rabbit polyclonal to ZCCHC9
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-ZCCHC9 antibody
  • Description

    Rabbit polyclonal to ZCCHC9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: TTYNKRPLPA TSWEDMKKGS FEGTSQNLPK RKQLEANRLS LKNDAPQAKH KKNKKKKEYL NEDVNGFMEY LRQNSQMVHN GQI, corresponding to N terminal amino acids 9-91 of Human ZCCHC9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Notes
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities

    Contains 4 CCHC-type zinc fingers.
  • Target information above from: UniProt accession Q8N567 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84240 Human
    • SwissProt: Q8N567 Human
    • Unigene: 15536 Human
    • Alternative names

      • PPP1R41 antibody
      • Protein phosphatase 1 regulatory subunit 41 antibody
      • Zcchc9 antibody
      • ZCHC9_HUMAN antibody
      • Zinc finger CCHC domain containing protein 9 antibody
      • Zinc finger CCHC domain-containing protein 9 antibody
      • Zinc finger, CCHC domain containing 9 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
    • Western blot - Anti-ZCCHC9 antibody (ab122470)
      Western blot - Anti-ZCCHC9 antibody (ab122470)
      All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution

      Lane 1 : Negative control (vector only transfected HEK293T lysate)
      Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

      Developed using the ECL technique.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab122470? Please let us know so that we can cite the reference in this datasheet.

    ab122470 has not yet been referenced specifically in any publications.

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