Overview

  • Product name
    Anti-ZCCHC9 antibody
  • Description
    Rabbit polyclonal to ZCCHC9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: TTYNKRPLPA TSWEDMKKGS FEGTSQNLPK RKQLEANRLS LKNDAPQAKH KKNKKKKEYL NEDVNGFMEY LRQNSQMVHN GQI, corresponding to N terminal amino acids 9-91 of Human ZCCHC9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Contains 4 CCHC-type zinc fingers.
  • Information by UniProt
  • Database links
    • Alternative names
      • PPP1R41 antibody
      • Protein phosphatase 1 regulatory subunit 41 antibody
      • Zcchc9 antibody
      • ZCHC9_HUMAN antibody
      • Zinc finger CCHC domain containing protein 9 antibody
      • Zinc finger CCHC domain-containing protein 9 antibody
      • Zinc finger, CCHC domain containing 9 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
    • Western blot - Anti-ZCCHC9 antibody (ab122470)
      Western blot - Anti-ZCCHC9 antibody (ab122470)
      All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution

      Lane 1 : Negative control (vector only transfected HEK293T lysate)
      Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

      Developed using the ECL technique.

    References

    ab122470 has not yet been referenced specifically in any publications.

    Publishing research using ab122470? Please let us know so that we can cite the reference in this datasheet.

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