Anti-ZCCHC9 antibody (ab122470)
Key features and details
- Rabbit polyclonal to ZCCHC9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZCCHC9 antibody -
Description
Rabbit polyclonal to ZCCHC9 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: TTYNKRPLPA TSWEDMKKGS FEGTSQNLPK RKQLEANRLS LKNDAPQAKH KKNKKKKEYL NEDVNGFMEY LRQNSQMVHN GQI, corresponding to N terminal amino acids 9-91 of Human ZCCHC9.
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Positive control
- Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
| Notes |
|---|
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Sequence similarities
Contains 4 CCHC-type zinc fingers. - Information by UniProt
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Database links
- Entrez Gene: 84240 Human
- SwissProt: Q8N567 Human
- Unigene: 15536 Human
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Alternative names
- PPP1R41 antibody
- Protein phosphatase 1 regulatory subunit 41 antibody
- Zcchc9 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry. -
Western blot - Anti-ZCCHC9 antibody (ab122470)All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)
Developed using the ECL technique.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122470 has not yet been referenced specifically in any publications.