Product nameAnti-ZCCHC9 antibody
DescriptionRabbit polyclonal to ZCCHC9
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesContains 4 CCHC-type zinc fingers.
- Information by UniProt
- PPP1R41 antibody
- Protein phosphatase 1 regulatory subunit 41 antibody
- Zcchc9 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)
Developed using the ECL technique.
ab122470 has not yet been referenced specifically in any publications.