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    zch11-antibody-ab244409.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-ZCH11 antibody (ab244409)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-ZCH11 antibody (ab244409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCH11 antibody (ab244409)

Key features and details

  • Rabbit polyclonal to ZCH11
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-ZCH11 antibody
    See all ZCH11 primary antibodies
  • Description

    Rabbit polyclonal to ZCH11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human ZCH11 aa 833-909.
    Sequence:

    CNCIDLSKSPDPDKSTGTDCRSNLETESSHQSVCTDTSATSCNCKATEDA SDLNDDDNLPTQELYYVFDKFILTSGK


    Database link: Q5TAX3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: U-251 MG cells. IHC-P: Human smooth muscle tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Our Abpromise guarantee covers the use of ab244409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
  • Sequence similarities

    Belongs to the DNA polymerase type-B-like family.
    Contains 3 CCHC-type zinc fingers.
    Contains 2 PAP-associated domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.
  • Target information above from: UniProt accession Q5TAX3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23318 Human
    • Omim: 613692 Human
    • SwissProt: Q5TAX3 Human
    • Unigene: 655407 Human
    • Alternative names

      • DKFZp779C1943 antibody
      • EC 2.7.7.52 antibody
      • FLJ42878 antibody
      • KIAA0191 antibody
      • PAP associated domain containing 3 antibody
      • PAPD3 antibody
      • Terminal uridylyltransferase 4 antibody
      • TUT4 antibody
      • TUT4_HUMAN antibody
      • TUTase 4 antibody
      • ZCCHC 11 antibody
      • Zcchc11 antibody
      • Zinc finger CCHC domain containing 11 antibody
      • Zinc finger CCHC domain containing protein 11 antibody
      • Zinc finger CCHC domain-containing protein 11 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ZCH11 antibody (ab244409)
      Immunocytochemistry/ Immunofluorescence - Anti-ZCH11 antibody (ab244409)

      PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for ZCH11 (green) using ab244409 at 4 µg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCH11 antibody (ab244409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCH11 antibody (ab244409)

      Formalin-fixed, paraffin-embedded human smooth muscle tissue stained for ZCH11 with ab244409 at a 1/20 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab244409? Please let us know so that we can cite the reference in this datasheet.

    ab244409 has not yet been referenced specifically in any publications.

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