Anti-ZCH11 antibody (ab244409)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ZCH11 antibody
See all ZCH11 primary antibodies -
Description
Rabbit polyclonal to ZCH11 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ZCH11 aa 833-909.
Sequence:CNCIDLSKSPDPDKSTGTDCRSNLETESSHQSVCTDTSATSCNCKATEDA SDLNDDDNLPTQELYYVFDKFILTSGK
Database link: Q5TAX3 -
Positive control
- ICC/IF: U-251 MG cells. IHC-P: Human smooth muscle tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab244409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
Sequence similarities
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
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Database links
- Entrez Gene: 23318 Human
- Omim: 613692 Human
- SwissProt: Q5TAX3 Human
- Unigene: 655407 Human
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Alternative names
- DKFZp779C1943 antibody
- EC 2.7.7.52 antibody
- FLJ42878 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ZCH11 antibody (ab244409)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for ZCH11 (green) using ab244409 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCH11 antibody (ab244409)Formalin-fixed, paraffin-embedded human smooth muscle tissue stained for ZCH11 with ab244409 at a 1/20 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab244409 has not yet been referenced specifically in any publications.
