Product nameZCH11 overexpression 293T lysate (whole cell)
ab94144 is a 293T cell transfected lysate in which Human ZCH11 has been transiently over-expressed using a pCMV-ZCH11 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
BackgroundFunction: Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Similarity: Belongs to the DNA polymerase type-B-like family. Contains 3 CCHC-type zinc fingers. Contains 2 PAP-associated domains. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
Our Abpromise guarantee covers the use of ab94144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94144 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-ZCH11 antibody (ab89165) at 1/500 dilution
Lane 1 :
ZCH11 overexpression 293T lysate (whole cell) (ab94144)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94144 has not yet been referenced specifically in any publications.