Anti-ZDHHC1 antibody (ab223042)
Key features and details
- Rabbit polyclonal to ZDHHC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ZDHHC1 antibody -
Description
Rabbit polyclonal to ZDHHC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ZDHHC1 aa 250-400.
Database link: Q8WTX9 -
Positive control
- WB: Mouse spleen tissue lysate. IHC-P: Human prostate cancer tissue. ICC/IF: A549 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
|
|
IHC-P |
1/20 - 1/200.
|
|
ICC/IF |
1/50 - 1/200.
|
Notes |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 55 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
-
Tissue specificity
Expressed at high levels in fetal lung, kidney and heart. Expressed at lower levels in adult pancreas and lung. -
Sequence similarities
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
Domain
The DHHC domain is required for palmitoyltransferase activity. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 29800 Human
- Entrez Gene: 70796 Mouse
- SwissProt: Q8WTX9 Human
- SwissProt: Q8R0N9 Mouse
- Unigene: 624541 Human
-
Alternative names
- C16orf1 antibody
- DHHC domain containing cysteine rich protein antibody
- DHHC domain-containing cysteine-rich protein 1 antibody
see all
Images
-
Anti-ZDHHC1 antibody (ab223042) at 1/1000 dilution + Mouse spleen tissue lysate
Secondary
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Paraffin-embedded human prostate cancer tissue stained for ZDHHC1 using ab223042 at 1/100 dilution in immunohistochemical analysis.
-
A549 (human lung carcinoma cell line) cells stained for ZDHHC1 (green) using ab223042 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab223042 has been referenced in 1 publication.
- Tang J et al. Cancer cells escape p53's tumor suppression through ablation of ZDHHC1-mediated p53 palmitoylation. Oncogene 40:5416-5426 (2021). PubMed: 34282274