Product nameAnti-ZDHHC14 antibody
See all ZDHHC14 primary antibodies
DescriptionRabbit polyclonal to ZDHHC14
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Recombinant fragment corresponding to Human ZDHHC14 aa 328-453.
Database link: Q8IZN3
- WB: HepG2, HEK-293, Jurkat and A549 whole cell lysates. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237503 in the following tested applications.
|ICC/IF||1/200 - 1/500.|
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 53 kDa.|
RelevanceZDHHC14 belongs to the DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily. It contains 1 DHHC type zinc finger. The function of ZDHHC14 remains unknown.
Cellular localizationMembrane; Multi pass membrane protein.
- DHHC 14 antibody
- NEW1 domain containing protein antibody
- NEW1CP antibody
All lanes : Anti-ZDHHC14 antibody (ab237503) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Paraffin-embedded human colon cancer tissue stained for ZDHHC14 using ab237503 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ZDHHC14 (green) using ab237503 at 1/200 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
ab237503 has not yet been referenced specifically in any publications.