Key features and details
- Rabbit polyclonal to ZDHHC14
- Suitable for: ICC/IF, IHC-P, WB
- Isotype: IgG
Product nameAnti-ZDHHC14 antibody
See all ZDHHC14 primary antibodies
DescriptionRabbit polyclonal to ZDHHC14
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Recombinant fragment corresponding to Human ZDHHC14 aa 328-453.
Database link: Q8IZN3
- WB: HepG2, HEK-293, Jurkat and A549 whole cell lysates. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.|
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 53 kDa.|
RelevanceZDHHC14 belongs to the DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily. It contains 1 DHHC type zinc finger. The function of ZDHHC14 remains unknown.
Cellular localizationMembrane; Multi pass membrane protein.
- DHHC 14 antibody
- NEW1 domain containing protein antibody
- NEW1CP antibody
All lanes : Anti-ZDHHC14 antibody (ab237503) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Paraffin-embedded human colon cancer tissue stained for ZDHHC14 using ab237503 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ZDHHC14 (green) using ab237503 at 1/200 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237503 has not yet been referenced specifically in any publications.