Overview

  • Product name

  • Description

    Rabbit polyclonal to ZDHHC14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Immunogen

    Recombinant fragment corresponding to Human ZDHHC14 aa 328-453.
    Database link: Q8IZN3

  • Positive control

    • WB: HepG2, HEK-293, Jurkat and A549 whole cell lysates. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.
IHC-P 1/500 - 1/1000.
WB 1/500 - 1/5000. Predicted molecular weight: 53 kDa.

Target

  • Relevance

    ZDHHC14 belongs to the DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily. It contains 1 DHHC type zinc finger. The function of ZDHHC14 remains unknown.
  • Cellular localization

    Membrane; Multi pass membrane protein.
  • Alternative names

    • DHHC 14 antibody
    • NEW1 domain containing protein antibody
    • NEW1CP antibody
    • Zinc finger DHHC domain containing 14 antibody
    • Zinc finger DHHC domain containing protein 14 antibody
    • zinc finger DHHC type containing 14 antibody
    see all

Images

  • All lanes : Anti-ZDHHC14 antibody (ab237503) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 53 kDa

  • Paraffin-embedded human colon cancer tissue stained for ZDHHC14 using ab237503 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for ZDHHC14 (green) using ab237503 at 1/200 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

References

ab237503 has not yet been referenced specifically in any publications.

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