The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/250.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Palmitoyltransferase specific for GAP43 and DLG4/PSD95.
Expressed in placenta, liver, lung, kidney, heart and brain.
Involvement in disease
Defects in ZDHHC15 are the cause of mental retardation X-linked type 91 (MRX91) [MIM:300577]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Belongs to the DHHC palmitoyltransferase family. Contains 1 DHHC-type zinc finger.
The DHHC domain is required for palmitoyltransferase activity.