Overview

  • Product name
    Anti-ZDHHC2 antibody - N-terminal
  • Description
    Rabbit polyclonal to ZDHHC2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ZDHHC2 aa 84-114 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UIJ5

  • Positive control
    • Neuro-2a cells. Human brain tissue. Ramos cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.
WB 1/100 - 1/500.

Target

  • Function
    Palmitoyltransferase specific for GAP43 and DLG4/PSD95.
  • Tissue specificity
    Ubiquitously expressed. Reduced expression in colorectal cancers with liver metastasis.
  • Involvement in disease
    Note=Mutations in ZDHHC2 are found in hepatocellular carcinoma and colorectal cancer.
  • Sequence similarities
    Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • Domain
    The DHHC domain is required for palmitoyltransferase activity.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHHC-2 antibody
    • Palmitoyltransferase ZDHHC2 antibody
    • Ream antibody
    • Rec antibody
    • Reduced expression associated with metastasis protein antibody
    • Reduced expression in cancer protein antibody
    • ZDHC2_HUMAN antibody
    • Zdhhc2 antibody
    • Zinc finger DHHC domain-containing protein 2 antibody
    • Zinc finger protein 372 antibody
    • ZNF372 antibody
    see all

Images

  • Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram) labeling ZDHHC2 with ab174967 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue labeling ZDHHC2 with ab174967 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-ZDHHC2 antibody - N-terminal (ab174967) at 1/60 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Ramos cell lysate with Blocking peptide
  • Anti-ZDHHC2 antibody - N-terminal (ab174967) at 1/100 dilution + Neuro-2a cell lysate at 15 µg

References

ab174967 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174967.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up