Key features and details
- Rabbit polyclonal to ZDHHC9
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ZDHHC9 antibody
See all ZDHHC9 primary antibodies
DescriptionRabbit polyclonal to ZDHHC9
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from C-terminal region of human ZDHHC9.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionThe ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.
Tissue specificityHighly expressed in kidney, skeletal muscle, brain, lung and liver. Absent in thymus, spleen and leukocytes.
Involvement in diseaseDefects in ZDHHC9 are the cause of mental retardation syndromic X-linked ZDHHC9-related (MRXSZ) [MIM:300799]. A disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Some patients have marfanoid habitus as an additional feature.
Sequence similaritiesBelongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
Contains 1 DHHC-type zinc finger.
DomainThe DHHC domain is required for palmitoyltransferase activity.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
- Antigen MMSA 1 antibody
- Asp His His Cys domain containing protein 9 antibody
- CGI89 antibody
All lanes : Anti-ZDHHC9 antibody (ab74504) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 26 kDa, 34 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab74504. Left: untreated sample. Right: sample treated with immunising peptide.
ab74504 has been referenced in 1 publication.
- Cambronne XA et al. Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach. Proc Natl Acad Sci U S A 109:20473-8 (2012). WB . PubMed: 23184980